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Authors Yang, CX; Zhao, WN; Wang, YN; Zhang, L; Huang, SC; Lin, JX
Author Full Name Yang, Chunxue; Zhao, Wenna; Wang, Yingnan; Zhang, Liang; Huang, Shouchen; Lin, Jixiang
Title Metabolomics Analysis Reveals the Alkali Tolerance Mechanism in Puccinellia tenuiflora Plants Inoculated with Arbuscular Mycorrhizal Fungi
Source MICROORGANISMS
Language English
Document Type Article
Author Keywords alkali stress; arbuscular mycorrhizal fungi; LC/TOF-MS; metabolomics; Puccinellia tenuiflora
Keywords Plus LEYMUS-CHINENSIS SEEDLINGS; SALT-STRESS; OSMOTIC ADJUSTMENT; DROUGHT STRESS; ORGANIC-ACIDS; IONIC BALANCE; GROWTH; SALINITY; RESPONSES; L.
Abstract Soil alkalization is a major environmental threat that affects plant distribution and yield in northeastern China. Puccinellia tenuiflora is an alkali-tolerant grass species that is used for salt-alkali grassland restoration. However, little is known about the molecular mechanisms by which arbuscular mycorrhizal fungi (AMF) enhance P. tenuiflora responses to alkali stress. Here, metabolite profiling in P. tenuiflora seedlings with or without arbuscular mycorrhizal fungi (AMF) under alkali stress was conducted using liquid chromatography combined with time-of-flight mass spectrometry (LC/TOF-MS). The results showed that AMF colonization increased seedling biomass under alkali stress. In addition, principal component analysis (PCA) and orthogonal projections to latent structures discriminant analysis (OPLS-DA) demonstrated that non-AM and AM seedlings showed different responses under alkali stress. A heat map analysis showed that the levels of 88 metabolites were significantly changed in non-AM seedlings, but those of only 31 metabolites were significantly changed in AM seedlings. Moreover, the levels of a total of 62 metabolites were significantly changed in P. tenuiflora seedlings after AMF inoculation. The results suggested that AMF inoculation significantly increased amino acid, organic acid, flavonoid and sterol contents to improve osmotic adjustment and maintain cell membrane stability under alkali stress. P. tenuiflora seedlings after AMF inoculation produced more plant hormones (salicylic acid and abscisic acid) than the non-AM seedlings, probably to enhance the antioxidant system and facilitate ion balance under stress conditions. In conclusion, these findings provide new insights into the metabolic mechanisms of P. tenuiflora seedlings with arbuscular mycorrhizal fungi under alkali conditions and clarify the role of AM in the molecular regulation of this species under alkali stress.
Author Address [Yang, Chunxue; Zhao, Wenna; Wang, Yingnan; Zhang, Liang; Huang, Shouchen; Lin, Jixiang] Northeast Forestry Univ, Coll Landscape Architecture, Harbin 150040, Peoples R China; [Lin, Jixiang] Northeast Forestry Univ, Key Lab Saline Alkali Vegetat Ecol Restorat, Minist Educ, Harbin 150040, Peoples R China
Reprint Address Lin, JX (corresponding author), Northeast Forestry Univ, Coll Landscape Architecture, Harbin 150040, Peoples R China.; Lin, JX (corresponding author), Northeast Forestry Univ, Key Lab Saline Alkali Vegetat Ecol Restorat, Minist Educ, Harbin 150040, Peoples R China.
E-mail Address yangchunxue121@foxmail.com; zwn9426@foxmail.com; yingnan1027@foxmail.com; z1978103903@foxmail.com; suthenyale@foxmail.com; linjixiang@nefu.edu.cn
ORCID Number Lin, jixiang/0000-0003-4367-8439; wang, yingnan/0000-0001-7138-7204
Funding Agency and Grant Number National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31601986, 31502013]; Fundamental Research Funds for the Central UniversitiesFundamental Research Funds for the Central Universities [2572018BK02]; Heilongjiang Postdoctoral Scientific Research Developmental Fund [LBH-Q16005]
Funding Text The research was supported by the National Natural Science Foundation of China (31601986, 31502013), Fundamental Research Funds for the Central Universities (2572018BK02), and Heilongjiang Postdoctoral Scientific Research Developmental Fund (LBH-Q16005).
Times Cited 2
Total Times Cited Count (WoS, BCI, and CSCD) 2
Publisher MDPI
Publisher City BASEL
Publisher Address ST ALBAN-ANLAGE 66, CH-4052 BASEL, SWITZERLAND
29-Character Source Abbreviation MICROORGANISMS
ISO Source Abbreviation Microorganisms
Publication Date MAR
Year Published 2020
Volume 8
Issue 3
Article Number 327
Digital Object Identifier (DOI) 10.3390/microorganisms8030327
Page Count 20
Web of Science Category Microbiology
Subject Category Microbiology
Document Delivery Number LD3SO
Unique Article Identifier WOS:000525952700015
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