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Authors Ozturk, SE; Goktay, M; Has, C; Babaoglu, M; Allmer, J; Doganlar, S; Frary, A
Author Full Name Ozturk, Saniye Elvan; Goktay, Mehmet; Has, Canan; Babaoglu, Mehmet; Allmer, Jens; Doganlar, Sami; Frary, Anne
Title Transcriptomic analysis of boron hyperaccumulation mechanisms in Puccinellia distans
Source CHEMOSPHERE
Language English
Document Type Article
Author Keywords Bioremediation; Boron; ESTs; Hyperaccumulation; Puccinellia distans; RNA sequencing
Keywords Plus BURP-DOMAIN PROTEIN; ABSCISIC-ACID; TOXICITY TOLERANCE; TRANSPORT MECHANISMS; GENE-EXPRESSION; STRESS-RESPONSE; SALT TOLERANCE; DROUGHT; OVEREXPRESSION; ACCUMULATION
Abstract Puccinellia distans, common alkali grass, is found throughout the world and can survive in soils with boron concentrations that are lethal for other plant species. Indeed, P. distans accumulates very high levels of this element. Despite these interesting features, very little research has been performed to elucidate the boron tolerance mechanism in this species. In this study, P. distans samples were treated for three weeks with normal (0.5 mg L-1) and elevated (500 mg L-1) boron levels in hydroponic solution. Expressed sequence tags (ESTs) derived from shoot tissue were analyzed by RNA sequencing to identify genes up and down-regulated under boron stress. In this way, 3312 differentially expressed transcripts were detected, 67.7% of which were up-regulated and 32.3% of which were down-regulated' in boron treated plants. To partially confirm the RNA sequencing results, 32 randomly selected transcripts were analyzed for their expression levels in boron-treated plants. The results agreed with the expected direction of change (up or down-regulation). A total of 1652 transcripts had homologs in A. thaliana and/or O. sativa and mapped to 1107 different proteins. Functional annotation of these proteins indicated that the boron tolerance and hyperaccumulation mechanisms of P. distans involve many transcriptomic changes including: alterations in the malate pathway, changes in cell wall components that may allow sequestration of excess boron without toxic effects, and increased expression of at least one putative boron transporter and two putative aquaporins. Elucidation of the boron accumulation mechanism is important in developing approaches for bioremediation of boron contaminated soils. (C) 2018 Elsevier Ltd. All rights reserved.
Author Address [Ozturk, Saniye Elvan; Goktay, Mehmet; Has, Canan; Allmer, Jens; Doganlar, Sami; Frary, Anne] Izmir Inst Technol, Dept Mol Biol & Genet, TR-35430 Izmir, Turkey; [Babaoglu, Mehmet] Selcuk Univ, Dept Field Crops, TR-42030 Selcuklu, Konya, Turkey
Reprint Address Frary, A (corresponding author), Izmir Inst Technol, Dept Mol Biol & Genet, TR-35430 Izmir, Turkey.
E-mail Address annefrary@iyte.edu.tr
ResearcherID Number Frary, Anne/A-5417-2015; OZTURK, Saniye Elvan/Q-2750-2018
ORCID Number Frary, Anne/0000-0002-8973-0100;
Funding Agency and Grant Number Scientific and Technological Research Council of TurkeyTurkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [113Z930]
Funding Text We thank Amy Frary for critical reading of the manuscript. This work was supported by a grant from The Scientific and Technological Research Council of Turkey (project no. 113Z930).
Times Cited 6
Total Times Cited Count (WoS, BCI, and CSCD) 6
Publisher PERGAMON-ELSEVIER SCIENCE LTD
Publisher City OXFORD
Publisher Address THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND
ISSN 0045-6535
29-Character Source Abbreviation CHEMOSPHERE
ISO Source Abbreviation Chemosphere
Publication Date MAY
Year Published 2018
Volume 199
Beginning Page 390
Ending Page 401
Digital Object Identifier (DOI) 10.1016/j.chemosphere.2018.02.070
Page Count 12
Web of Science Category Environmental Sciences
Subject Category Environmental Sciences & Ecology
Document Delivery Number GB3QT
Unique Article Identifier WOS:000428973200047
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