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Authors Eilmus, S; Rosch, C; Bothe, H
Author Full Name Eilmus, Sascha; Roesch, Christopher; Bothe, Hermann
Title Prokaryotic life in a potash-polluted marsh with emphasis on N-metabolizing microorganisms
Source ENVIRONMENTAL POLLUTION
Language English
Document Type Article
Author Keywords prokaryotic community analysis; computer program for assessing biodiversity; bacteria in saline soils; denitrification; dinitrogen fixation; nitrification; bacteria in potash mining waste
Keywords Plus 16S RIBOSOMAL-RNA; MOLECULAR MICROBIAL DIVERSITY; BACTERIAL COMMUNITY; SOIL; CULTIVATION; SEQUENCES; PROFILES; GRADIENT; ARCHAEA; ROOTS
Abstract Prokaryotic life along the salt gradient of the potash marsh resulting from mining waste at Schreyahn, Northern Germany, was screened for the distribution of total prokaryote (assessed by the 16S rRNA gene) and of N-2-fixing (nifH gene), denitrifying (nosZ) and nitrifying (amoA) microorganisms. Information on prokaryotes was retrieved from the different soil sites (a) by culturing in conventional media, (b) by isolating the DNA, amplifying the target genes by PCR followed by sequencing, (c) by employing the recently developed computer program (TReFID [Rosch, C., Bothe, H., 2005. Improved assessment of denitrifying, N2-fixing, and total-community bacteria by terminal restriction fragment length polymorphism analysis using multiple restriction enzymes. Applied and Environmental Microbiology 71, 2026-2035]) based on tRFLP data. New sequences were obtained as well as ones that were almost identical to those found at far distant locations. Whereas the distribution of plants strictly follows the salt gradient, this is apparently not the case with prokaryotes. Bacteria of hypersaline areas coexist with salt-non-tolerant species. The recently developed TReFID program is successfully applied to characterize a prokaryote community structure. (c) 2006 Elsevier Ltd. All rights reserved.
Author Address Univ Cologne, Inst Bot, D-50923 Cologne, Germany
Reprint Address Bothe, H (corresponding author), Univ Cologne, Inst Bot, Gyrhofstr 15, D-50923 Cologne, Germany.
E-mail Address hermann.bothe@uni-koeln.de
Times Cited 13
Total Times Cited Count (WoS, BCI, and CSCD) 14
Publisher ELSEVIER SCI LTD
Publisher City OXFORD
Publisher Address THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND
ISSN 0269-7491
29-Character Source Abbreviation ENVIRON POLLUT
ISO Source Abbreviation Environ. Pollut.
Publication Date MAR
Year Published 2007
Volume 146
Issue 2
Beginning Page 478
Ending Page 491
Digital Object Identifier (DOI) 10.1016/j.envpol.2006.07.008
Page Count 14
Web of Science Category Environmental Sciences
Subject Category Environmental Sciences & Ecology
Document Delivery Number 146YR
Unique Article Identifier WOS:000244971100025
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