Loading content, please wait..
loading..
Logo
Version 3.21
or
Authors George, S; Venkataraman, G; Parida, A
Author Full Name George, Suja; Venkataraman, Gayatri; Parida, Ajay
Title Identification of stress-induced genes from the drought-tolerant plant Prosopis juiliflora (Swartz) DC. through analysis of expressed sequence tags
Source GENOME
Language English
Document Type Article
Author Keywords drought stress; Prosopis juliflora; ESTs; reverse Northern
Keywords Plus LIPID TRANSFER PROTEIN; MOLECULAR RESPONSES; ARABIDOPSIS; SALT; COLD; PHOSPHATASE; RESISTANCE; INDUCTION; JULIFLORA; ALIGNMENT
Abstract Abiotic stresses such as cold, salinity, drought, wounding, and heavy metal contamination adversely affect crop productivity throughout the world. Prosopis juliflora is a phreatophyte that can tolerate severe adverse environmental conditions such as drought, salinity, and heavy metal contamination. As a first step towards the characterization of genes that contribute to combating abiotic stress, construction and analysis of a cDNA library of P. juliflora genes is reported here. Random expressed sequence tag (EST) sequencing of 1750 clones produced 1467 high-quality reads. These clones were classified into functional categories, and BLAST comparisons revealed that 114 clones were homologous to genes implicated in stress response(s) and included heat shock proteins, metallothioneins, lipid transfer proteins, and late embryogenesis abundant proteins. Of the ESTs analyzed, 26% showed homology to previously uncharacterized genes in the databases. Fifty-two clones from this category were selected for reverse Northern analysis: 21 were shown to be upregulated and 16 downregulated. The results obtained by reverse Northern analysis were confirmed by Northern analysis. Clustering of the 1467 ESTs produced a total of 295 contigs encompassing 790 ESTs, resulting in a 54.2% redundancy. Two of the abundant genes coding for a nonspecific lipid transfer protein and late embryogenesis abundant protein were sequenced completely. Northern analysis (after polyethylene glycol stress) of the 2 genes was carried out. The implications of the analyzed genes in abiotic stress tolerance are also discussed.
Author Address MS Swaminathan Res Fdn, Dept Plant Mol Biol, Madras 600113, Tamil Nadu, India
Reprint Address Parida, A (corresponding author), MS Swaminathan Res Fdn, Dept Plant Mol Biol, Taramani Inst Area, Madras 600113, Tamil Nadu, India.
E-mail Address ajay@mssrf.res.in
ORCID Number , Gayatri/0000-0001-6926-3317
Times Cited 17
Total Times Cited Count (WoS, BCI, and CSCD) 19
Publisher CANADIAN SCIENCE PUBLISHING
Publisher City OTTAWA
Publisher Address 65 AURIGA DR, SUITE 203, OTTAWA, ON K2E 7W6, CANADA
ISSN 0831-2796
29-Character Source Abbreviation GENOME
ISO Source Abbreviation Genome
Publication Date MAY
Year Published 2007
Volume 50
Issue 5
Beginning Page 470
Ending Page 478
Digital Object Identifier (DOI) 10.1139/G07-014
Page Count 9
Web of Science Category Biotechnology & Applied Microbiology; Genetics & Heredity
Subject Category Biotechnology & Applied Microbiology; Genetics & Heredity
Document Delivery Number 197XU
Unique Article Identifier WOS:000248591300006
Plants associated with this reference

LEGAL NOTICES — This website is protected by Copyright © The University of Sussex, 2012, 2013, 2014, 2015, 2016, 2017, 2018, 2019, 2020, 2021. The eHALOPH database is protected by Database Right and Copyright © The University of Sussex and other contributors, 2006, 2012, 2013, 2014, 2015, 2016, 2017, 2018, 2019, 2020, 2021. This database is based on an earlier work by James Aronson.
THIS WEBSITE AND THIS DATABASE ARE PROVIDED ON AN "AS IS" BASIS, AND YOU USE THEM AND RELY ON THEM AT YOUR OWN RISK.

Contact email: halophytes@sussex.ac.uk
Credits – Tim Flowers, Joaquim Santos, Moritz Jahns, Brian Warburton, Peter Reed