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Authors Kulkarni, S; Nautiyal, CS
Author Full Name Kulkarni, S; Nautiyal, CS
Title Characterization of high temperature-tolerant rhizobia isolated from Prosopis juliflora grown in alkaline soil
Source JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY
Language English
Document Type Article
Author Keywords leguminous trees; Rhizobium; root nodules; stress tolerance
Keywords Plus NITROGEN-FIXATION; LEGUMINOUS TREES; SYMBIOTIC PERFORMANCE; SODIUM-CHLORIDE; ROOT-NODULES; STRAINS; RHIZOSPHERE; SENSITIVITY; INOCULATION; CASUARINA
Abstract A method was developed for the fast screening and selection of high-temperature tolerant rhizobial strains from root nodules of Prosopis juliflora growing in alkaline soils. The high-temperature tolerant rhizobia were selected from 2,500 Rhizobium isolates with similar growth patterns on yeast mannitol agar plates after 72 h incubation at 30 and 45 degrees C, followed by a second screening at 47.5 degrees C, Seventeen high-temperature tolerant rhizobial strains having distinguishable protein band patterns were finally selected for further screening by subjecting them to temperature stress up to 60 degrees C in yeast mannitol broth for 6 h. The high-temperature tolerant strains were NBRI12, NBRI329, NBRI330, NBRI332, and NBRI133. Using this procedure, a large number of rhizobia from root nodules of P. juliflora were screened for high-temperature tolerance. The assimilation of several carbon sources, tolerance to high pH and salt stress, and ability to nodulate P. juliflora growing in a glasshouse and nursery of the strains were studied. All five isolates had higher plant dry weight in the range of 29.9 to 88.6% in comparison with uninoculated nursery-grown plants. It was demonstrated that it is possible to screen in nature for superior rhizobia exemplified by the isolation of temperature-tolerant strains, which established effective symbiosis with nursery-grown P. juliflora. These findings indicate a correlation between strain performance under in vitro stress in pure culture and strain behavior under symbiotic conditions. Pure culture evaluation may be a useful tool in search for Rhizobium strains better suited for soil environments where high temperature, pH, and salt stress constitutes a limitation for symbiotic biological nitrogen fixation.
Author Address Natl Bot Res Inst, Microbiol Grp, Lucknow 226001, Uttar Pradesh, India
Reprint Address Nautiyal, CS (corresponding author), Natl Bot Res Inst, Microbiol Grp, Rana Pratag Marg, Lucknow 226001, Uttar Pradesh, India.
Times Cited 17
Total Times Cited Count (WoS, BCI, and CSCD) 18
Publisher MICROBIOL RES FOUNDATION
Publisher City TOKYO
Publisher Address JAPAN ACAD SOC CENTER BLDG 4-16 YAYOI 2-CHOME, TOKYO, 113-0032, JAPAN
ISSN 0022-1260
29-Character Source Abbreviation J GEN APPL MICROBIOL
ISO Source Abbreviation J. Gen. Appl. Microbiol.
Publication Date OCT
Year Published 1999
Volume 45
Issue 5
Beginning Page 213
Ending Page 220
Digital Object Identifier (DOI) 10.2323/jgam.45.213
Page Count 8
Web of Science Category Biotechnology & Applied Microbiology; Microbiology
Subject Category Biotechnology & Applied Microbiology; Microbiology
Document Delivery Number 302BV
Unique Article Identifier WOS:000086345800002
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