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Authors Rai, MK; Shekhawat, JK; Kataria, V; Shekhawat, NS
Author Full Name Rai, Manoj K.; Shekhawat, Jatan K.; Kataria, Vinod; Shekhawat, N. S.
Title Cross species transferability and characterization of microsatellite markers in Prosopis cineraria, a multipurpose tree species of Indian Thar Desert
Source ARID LAND RESEARCH AND MANAGEMENT
Language English
Document Type Article
Author Keywords Cross-species transferability; microsatellite markers; Prosopis cineraria; polymorphism
Keywords Plus SSR MARKERS; GENETIC DIVERSITY; L.; RAPD; AMPLIFICATION; LEGUMINOSAE; LIMITATIONS; MYRTACEAE; PLOIDY; DRUCE
Abstract Prosopis cineraria (L.) Druce, family Fabaceae, is an important multipurpose tree species of the Indian Thar Desert. It is an extremely drought and heat tolerant plant and has ability to survive in highly alkaline and saline environments. Considering the commercial, horticultural and ecological importance of P. cineraria, there is a great need to develop genomic resources in the form of microsatellite markers for the genetic enhancement of this crop. We identified and characterized 10 microsatellite markers in P. cineraria by cross species amplification. Total 18 Simple Sequence Repeat (SSR) primer pairs developed in P. chilensis, P. alba, and P. flexuosa were used to amplify SSR loci in P. cineraria. Out of eighteen SSR markers tested, ten (55.5%) amplified recognizable amplicons. The number of alleles detected at each locus ranged from one to four, a total of 24 with an average of 2.4 alleles. Observed heterozygosity (Ho) and expected heterozygosity (He) values varied from 0.14 to 0.85 and 0.21 to 0.56 with an average of 0.47 and 0.37, respectively. The polymorphic information content (PIC) values ranged from 0.49 to 0.78 with an average of 0.66. Of the nine polymorphic markers, seven were highly informative and polymorphic (PIC > 0.5). These microsatellite markers are characterized for the first time in P. cineraria. All microsatellite markers identified in this study may be useful in comparative genomics and population genetics studies of P. cineraria.
Author Address [Rai, Manoj K.; Shekhawat, Jatan K.; Kataria, Vinod; Shekhawat, N. S.] Jai Narain Vyas Univ, Dept Bot, Biotechnol Unit, Jodhpur, Rajasthan, India; [Rai, Manoj K.] Indira Gandhi Natl Tribal Univ, Dept Environm Sci, Amarkantak 484887, MP, India
Reprint Address Rai, MK (corresponding author), Indira Gandhi Natl Tribal Univ, Dept Environm Sci, Amarkantak 484887, MP, India.
E-mail Address mkraibhu@gmail.com
ResearcherID Number Rai, Manoj K/F-3104-2012
ORCID Number Rai, Manoj K/0000-0002-2385-6325; Kataria, Vinod/0000-0001-5659-6374
Funding Agency and Grant Number Science and Engineering Research Board (SERB), New Delhi [SB/YS/LS-292/2013]; UGC, New DelhiUniversity Grants Commission, India; Junior Research Fellowship (UGC-JRF)
Funding Text The financial support provided by the Science and Engineering Research Board (SERB), New Delhi to M. K. Rai under the scheme
Times Cited 4
Total Times Cited Count (WoS, BCI, and CSCD) 4
Publisher TAYLOR & FRANCIS INC
Publisher City PHILADELPHIA
Publisher Address 530 WALNUT STREET, STE 850, PHILADELPHIA, PA 19106 USA
ISSN 1532-4982
29-Character Source Abbreviation ARID LAND RES MANAG
ISO Source Abbreviation Arid Land Res. Manag.
Year Published 2017
Volume 31
Issue 4
Beginning Page 462
Ending Page 471
Digital Object Identifier (DOI) 10.1080/15324982.2017.1338791
Page Count 10
Web of Science Category Environmental Sciences; Soil Science
Subject Category Environmental Sciences & Ecology; Agriculture
Document Delivery Number FP2WN
Unique Article Identifier WOS:000417480800007
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