Loading content, please wait..
loading..
Logo
Version 3.22
or
Authors Kumari, J; Udawat, P; Dubey, AK; Haquo, MI; Rathore, MS; Jha, B
Author Full Name Kumari, Jyoti; Udawat, Pushpika; Dubey, Ashish K.; Haquo, Md Intesaful; Rathore, Mangal S.; Jha, Bhavanath
Title Overexpression of SbSI-1, A Nuclear Protein from Salicornia brachiata Confers Drought and Salt Stress Tolerance and Maintains Photosynthetic Efficiency in Transgenic Tobacco
Source FRONTIERS IN PLANT SCIENCE
Language English
Document Type Article
Author Keywords abiotic stress; drought; halophyte; oxidative damage; salt; and transgenic
Keywords Plus EXPRESSED SEQUENCE TAGS; SUPEROXIDE-DISMUTASE; ABIOTIC STRESS; ANTIOXIDANT MACHINERY; ECTOPIC EXPRESSION; HYDROGEN-PEROXIDE; TISSUE CULTURES; COLD TOLERANCE; INDUCED GENES; SALINITY
Abstract A novel (S) under bar alicornia (b) under bar rachiata (S) under bar alt (I) under bar nducible (SbSI-1) gene was isolated and overexpressed in tobacco for in planta functional validation subjected to drought and salt stress. SbSI-1 is a nuclear protein. The transgenic tobacco overexpressing SbSI-1 gene exhibited better seed germination, growth performances, pigment contents, cell viability, starch accumulation, and tolerance index under drought and salt stress. Overexpression of SbSI-1 gene alleviated the build-up of reactive oxygen species (ROS) and curtailed the ROS-induced oxidative damages thus improved the physiological health of transgenic tobacco under stressed conditions. The higher activities of antioxidant enzymes, lower accumulation of ROS, higher membrane stability, relative water content, and polyphenol contents indicated the better survival of the transgenic tobacco than wild-type (WT) tobacco under stressed conditions. Transgenic tobacco had a higher net photosynthetic rate, PSII operating efficiency, and performance index under drought and salt stress. Higher accumulation of compatible solutes and K+/Na+ ratio in transgenic tobacco than WT showed the better osmotic and redox homeostasis under stressed conditions. The up-regulation of genes encoding antioxidant enzymes (NtSOD, NtAPX, and NtCAT) and transcription factors (NtDREB2 and NtAP2) in transgenic tobacco under stressed conditions showed the role of SbSI-1 in ROS alleviation and involvement of this gene in abiotic stress tolerance. Multivariate data analysis exhibited statistical distinction among growth responses, physiological health, osmotic adjustment, and photosynthetic responses of WT and transgenic tobacco under stressed conditions. The overexpression of SbSI-1 gene curtailed the ROS-induced oxidative damages and maintained the osmotic homeostasis under stress conditions thus improved physiological health and photosynthetic efficiencies of the transgenic tobacco overexpressing SbSI-1 gene.
Author Address [Kumari, Jyoti; Udawat, Pushpika; Dubey, Ashish K.; Haquo, Md Intesaful; Rathore, Mangal S.; Jha, Bhavanath] CSIR, Cent Salt & Marine Chem Res Inst, Marine Biotechnol & Ecol Div, Bhavnagar, Gujarat, India; [Kumari, Jyoti; Udawat, Pushpika; Rathore, Mangal S.; Jha, Bhavanath] CSIR, Acad Sci & Innovat Res, New Delhi, India
Reprint Address Rathore, MS; Jha, B (corresponding author), CSIR, Cent Salt & Marine Chem Res Inst, Marine Biotechnol & Ecol Div, Bhavnagar, Gujarat, India.; Rathore, MS; Jha, B (corresponding author), CSIR, Acad Sci & Innovat Res, New Delhi, India.
E-mail Address mangalrathore@csmcri.res.in; bjha@csmcri.res.in
ORCID Number Rathore, Mangal S./0000-0001-5434-822X; Kumari, Jyoti/0000-0001-8055-2363
Funding Agency and Grant Number Council of Scientific and Industrial Research (CSIR), Government of India, New DelhiCouncil of Scientific & Industrial Research (CSIR) - India [BSC0109-SIMPLE]
Funding Text CSIR-CSMCRI Communication No.: PRIS-075/2016. The authors acknowledge the Council of Scientific and Industrial Research (CSIR; http://www.csir.res.in), Government of India, New Delhi [BSC0109-SIMPLE] the financial support to carry the study. The funders did not have any role in designing the study, experimentation, data analysis, decision to publish and preparation of the manuscript. The authors are thankful for the help of Dr. N. S. Yadav for gene cloning, Dr. V. K. Singh for gene localization study and Dr. A. Mishra for RT-PCR analysis.
Times Cited 15
Total Times Cited Count (WoS, BCI, and CSCD) 15
Publisher FRONTIERS MEDIA SA
Publisher City LAUSANNE
Publisher Address PO BOX 110, EPFL INNOVATION PARK, BUILDING I, LAUSANNE, 1015, SWITZERLAND
ISSN 1664-462X
29-Character Source Abbreviation FRONT PLANT SCI
ISO Source Abbreviation Front. Plant Sci.
Publication Date JUL 13
Year Published 2017
Volume 8
Article Number 1215
Digital Object Identifier (DOI) 10.3389/fpls.2017.01215
Page Count 18
Web of Science Category Plant Sciences
Subject Category Plant Sciences
Document Delivery Number FB3DP
Unique Article Identifier WOS:000406023100001
Plants associated with this reference

LEGAL NOTICES — This website is protected by Copyright © The University of Sussex, 2012, 2013, 2014, 2015, 2016, 2017, 2018, 2019, 2020, 2021. The eHALOPH database is protected by Database Right and Copyright © The University of Sussex and other contributors, 2006, 2012, 2013, 2014, 2015, 2016, 2017, 2018, 2019, 2020, 2021. This database is based on an earlier work by James Aronson.
THIS WEBSITE AND THIS DATABASE ARE PROVIDED ON AN "AS IS" BASIS, AND YOU USE THEM AND RELY ON THEM AT YOUR OWN RISK.

Contact email: halophytes@sussex.ac.uk
Credits – Tim Flowers, Joaquim Santos, Moritz Jahns, Brian Warburton, Peter Reed