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Authors Maestrini, P; Giordani, T; Lunardi, A; Cavallini, A; Natali, L
Author Full Name Maestrini, P; Giordani, T; Lunardi, A; Cavallini, A; Natali, L
Title Isolation and expression of two aquaporin-encoding genes from the marine phanerogam Posidonia oceanica
Source PLANT AND CELL PHYSIOLOGY
Language English
Document Type Article
Author Keywords aquaporin; marine plants; Posidonia oceanica; salinity; seagrass
Keywords Plus PLASMA-MEMBRANE AQUAPORINS; WATER CHANNEL ACTIVITY; TISSUE-SPECIFIC EXPRESSION; INTRINSIC PROTEINS; ION CHANNELS; SEAGRASS; PLANTS; INTERNODES; SEQUENCES; TRANSPORT
Abstract Seagrasses such as Posidonia oceanica (L.) Delile are marine phanerogams, widespread in various seas, where they form large prairies representing dynamic substrates exceeding the area of the sediment surface several times over and allowing settlement of epiphyte organisms. Studying mechanisms involved in water transport in marine plants, we isolated two aquaporin-encoding genes, PoPIP1;1 and PoTIP1;1, showing high similarity to plasma membrane- and tonoplast-intrinsic protein-encoding genes, respectively. PoPIP1;1 is unique in the genome of P. oceanica, while PoTIP1;1 belongs to an aquaporin subfamily of at least four members. PoPIP1;1 and PoTIP1;1 encode functional proteins, as indicated by expression experiments in Xenopus oocytes. Both genes are constitutively expressed in the leaves, with higher levels of transcripts in young than in differentiated leaf tissues. Variations of salt concentration in aquarium determined different PoPIP1;1 and PoTIP1;1 transcript accumulation, indicating the existence of adaptation mechanisms related to gene expression also in marine plants, i.e. adapted to very high salt concentrations. Hyposalinity induced lower levels of PIP1 transcripts, while hypersalinity determined more PIP1 transcripts than normal salinity. TIP1 transcripts increased in response to both hypo- and hypersalinity after 2 days of treatment and went back to control levels after 5 d.
Author Address Univ Pisa, Dept Agr Plant Biol, Genet Sect, I-56124 Pisa, Italy; Univ Pisa, Dept Physiol & Biochem, I-56127 Pisa, Italy
Reprint Address Natali, L (corresponding author), Univ Pisa, Dept Agr Plant Biol, Genet Sect, Via Matteotti 1-B, I-56124 Pisa, Italy.
E-mail Address lnatali@agr.unipi.it
ResearcherID Number Cavallini, Andrea/AAF-1812-2020
Times Cited 8
Total Times Cited Count (WoS, BCI, and CSCD) 10
Publisher OXFORD UNIV PRESS
Publisher City OXFORD
Publisher Address GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND
ISSN 0032-0781
29-Character Source Abbreviation PLANT CELL PHYSIOL
ISO Source Abbreviation Plant Cell Physiol.
Publication Date DEC
Year Published 2004
Volume 45
Issue 12
Beginning Page 1838
Ending Page 1847
Digital Object Identifier (DOI) 10.1093/pcp/pch213
Page Count 10
Web of Science Category Plant Sciences; Cell Biology
Subject Category Plant Sciences; Cell Biology
Document Delivery Number 889XK
Unique Article Identifier WOS:000226475900012
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