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Authors Serra, IA; Lauritano, C; Dattolo, E; Puoti, A; Nicastro, S; Innocenti, AM; Procaccini, G
Author Full Name Serra, Ilia Anna; Lauritano, Chiara; Dattolo, Emanuela; Puoti, Andrea; Nicastro, Silvia; Innocenti, Anna Maria; Procaccini, Gabriele
Title Reference genes assessment for the seagrass Posidonia oceanica in different salinity, pH and light conditions
Source MARINE BIOLOGY
Language English
Document Type Article
Keywords Plus REAL-TIME PCR; PLASMA-MEMBRANE AQUAPORINS; POLYMERASE-CHAIN-REACTION; RELIABLE REFERENCE GENES; QUANTITATIVE RT-PCR; NICOTIANA-TABACUM; EXPRESSION; SELECTION; NORMALIZATION; VALIDATION
Abstract The stability on the expression level of putative reference genes (RGs) for reverse transcriptase-quantitative PCR (RT-qPCR) was tested in the seagrass Posidonia oceanica for plants collected in different environmental conditions (at different depths and in different salinity and pH). Besides adult plants, seedlings were also used for pH and salinity experiments. The putative RGs encompassed the most frequently used in other species to normalize RT-qPCR in a range of experimental conditions. Assignment of the best RGs has been performed with three Excel-based applets, BestKeeper, geNorm and NormFinder, and the results showed that best RGs may change depending on the experimental conditions tested, plant age and on the software utilized. Preferred sets of RGs are proposed for each of the conditions and a general procedure concerning their selection is discussed. In addition, expression levels of four genes involved in plant response to light, salinity and pH variation have also been evaluated.
Author Address [Lauritano, Chiara; Dattolo, Emanuela; Puoti, Andrea; Procaccini, Gabriele] Stn Zool Anton Dohrn, I-80121 Naples, Italy; [Serra, Ilia Anna; Nicastro, Silvia; Innocenti, Anna Maria] Univ Calabria, I-87036 Cosenza, Italy
Reprint Address Procaccini, G (corresponding author), Stn Zool Anton Dohrn, I-80121 Naples, Italy.
E-mail Address gpro@szn.it
ResearcherID Number Dattolo, Emanuela/ABD-6535-2020; Procaccini, Gabriele/A-6618-2010; Procaccini, Gabriele/AAA-7040-2019; Lauritano, Chiara/Q-4166-2018
ORCID Number Dattolo, Emanuela/0000-0001-6005-5079; Procaccini, Gabriele/0000-0002-6179-468X; Procaccini, Gabriele/0000-0002-6179-468X; Lauritano, Chiara/0000-0003-4580-9594
Times Cited 29
Total Times Cited Count (WoS, BCI, and CSCD) 30
Publisher SPRINGER HEIDELBERG
Publisher City HEIDELBERG
Publisher Address TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY
ISSN 0025-3162
29-Character Source Abbreviation MAR BIOL
ISO Source Abbreviation Mar. Biol.
Publication Date JUN
Year Published 2012
Volume 159
Issue 6
Beginning Page 1269
Ending Page 1282
Digital Object Identifier (DOI) 10.1007/s00227-012-1907-8
Page Count 14
Web of Science Category Marine & Freshwater Biology
Subject Category Marine & Freshwater Biology
Document Delivery Number 934UL
Unique Article Identifier WOS:000303472100008
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