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Authors Garcias-Bonet, N; Arrieta, JM; Duarte, CM; Marba, N
Author Full Name Garcias-Bonet, Neus; Arrieta, Jesus M.; Duarte, Carlos M.; Marba, Nuria
Title Nitrogen-fixing bacteria in Mediterranean seagrass (Posidonia oceanica) roots
Source AQUATIC BOTANY
Language English
Document Type Article
Author Keywords Diazotrophy; Nitrogen fixation; Seagrass-bacteria interaction; Endophytes
Keywords Plus DIAZOTROPHIC BACTERIA; FIXATION; DIVERSITY; SEDIMENTS; MEADOWS; RICE; RHIZOSPHERE; ENVIRONMENT; ASSEMBLAGE; GROWTH
Abstract Biological nitrogen fixation by diazotrophic bacteria in seagrass rhizosphere and leaf epiphytic community is an important source of nitrogen required for plant growth. However, the presence of endophytic diazotrophs remains unclear in seagrass tissues. Here, we assess the presence, diversity and taxonomy of nitrogen-fixing bacteria within surface-sterilized roots of Posidonia oceanica. Moreover, we analyze the nitrogen isotopic signature of seagrass tissues in order to notice atmospheric nitrogen fixation. We detected nitrogen-fixing bacteria by ray gene amplification in 13 out of the 78 roots sampled, corresponding to 9 locations out of 26 meadows. We detected two different types of bacterial nifH sequences associated with P. oceanica roots, which were closely related to sequences previously isolated from the rhizosphere of a salt marsh cord grass and a putative anaerobe. Nitrogen content of seagrass tissues showed low isotopic signatures in all the sampled meadows, pointing out the atmospheric origin of the assimilated nitrogen by seagrasses. However, this was not related with the presence of endophytic nitrogen fixers, suggesting the nitrogen fixation occurring in rhizosphere and in the epiphytic community could be an important source of nitrogen for P. oceanica. The low diversity of nitrogen-fixing bacteria reported here suggests species-specific relationships between diazotrophs and P. oceanica, revealing possible symbiotic interactions that could play a major role in nitrogen acquisition by seagrasses in oligotrophic environments where they form lush meadows. (C) 2016 Elsevier B.V. All rights reserved.
Author Address [Garcias-Bonet, Neus; Arrieta, Jesus M.; Duarte, Carlos M.; Marba, Nuria] UIB, Dept Global Change Res, CSIC, Inst Mediterrani Estudis Avancats,IMEDEA, Esporles, Spain; [Garcias-Bonet, Neus; Arrieta, Jesus M.; Duarte, Carlos M.] King Abdullah Univ Sci & Technol KAUST, Red Sea Res Ctr RSRC, Thuwal 239556900, Saudi Arabia
Reprint Address Garcias-Bonet, N (corresponding author), King Abdullah Univ Sci & Technol KAUST, Red Sea Res Ctr RSRC, Div Biol & Environm Sci & Engn, Thuwal 239556900, Saudi Arabia.
E-mail Address neus.garciasbonet@kaust.edu.sa; jesus.arrieta@kaust.edu.sa; carlos.duarte@kaust.edu.sa; nmarba@imedea.uib-csic.es
ResearcherID Number Arrieta, Jesus M/B-1226-2008; Marba, Nuria/H-8136-2015; Duarte, Carlos M./A-7670-2013
ORCID Number Arrieta, Jesus M/0000-0002-0190-6950; Marba, Nuria/0000-0002-8048-6789; Duarte, Carlos M./0000-0002-1213-1361; Garcias-Bonet, Neus/0000-0001-6469-7167
Funding Agency and Grant Number project MEDEICG of Spanish Marine Science and Technology Program; project ESTRESX of the Spanish Marine Science and Technology Program [CTM2009-07013, CTM2012-32603]; PhD grant from Government of Balearic Islands [FPI04 43126005Q]
Funding Text This study was funded by the projects MEDEICG and ESTRESX of the Spanish Marine Science and Technology Program (CTM2009-07013, CTM2012-32603). N.G.B. was supported by a PhD grant from the Government of Balearic Islands (reference FPI04 43126005Q) and J.M.A. by a contract of the Ramon y Cajal program of the Spanish Ministry of Economy and Competitiveness.
Times Cited 19
Total Times Cited Count (WoS, BCI, and CSCD) 19
Publisher ELSEVIER SCIENCE BV
Publisher City AMSTERDAM
Publisher Address PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS
ISSN 0304-3770
29-Character Source Abbreviation AQUAT BOT
ISO Source Abbreviation Aquat. Bot.
Publication Date MAY
Year Published 2016
Volume 131
Beginning Page 57
Ending Page 60
Digital Object Identifier (DOI) 10.1016/j.aquabot.2016.03.002
Page Count 4
Web of Science Category Plant Sciences; Marine & Freshwater Biology
Subject Category Plant Sciences; Marine & Freshwater Biology
Document Delivery Number DK4VV
Unique Article Identifier WOS:000374919300008
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