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Authors Kearl, J; McNary, C; Lowman, JS; Mei, CS; Aanderud, ZT; Smith, ST; West, J; Colton, E; Hamson, M; Nielsen, BL
Author Full Name Kearl, Jennifer; McNary, Caitlyn; Lowman, J. Scott; Mei, Chuansheng; Aanderud, Zachary T.; Smith, Steven T.; West, Jason; Colton, Emily; Hamson, Michelle; Nielsen, Brent L.
Title Salt-Tolerant Halophyte Rhizosphere Bacteria Stimulate Growth of Alfalfa in Salty Soil
Source FRONTIERS IN MICROBIOLOGY
Language English
Document Type Article
Author Keywords halophyte; halophilic bacteria; rhizosphere; endophyte; microbiome; salt tolerance; plant growth-promoting rhizobacteria
Keywords Plus ENDOPHYTIC BACTERIA; PROMOTING BACTERIA; BIOFILM FORMATION; STRESS TOLERANCE; SEQUENCE DATA; GEN. NOV.; RHIZOBACTERIA; INOCULATION; STRATEGY; MEMBER
Abstract Halophytes are plants that are adapted to grow in saline soils, and have been widely studied for their physiological and molecular characteristics, but little is known about their associated microbiomes. Bacteria were isolated from the rhizosphere and as root endophytes of Salicomia rubra, Sarcocomia utahensis, and Allenrolfea occidentalis, three native Utah halophytes. A total of 41 independent isolates were identified by 16S rRNA gene sequencing analysis. Isolates were tested for maximum salt tolerance, and some were able to grow in the presence of up to 4 M NaCl. Pigmentation, Gram stain characteristics, optimal temperature for growth, and biofilm formation of each isolate aided in species identification. Some variation in the bacterial population was observed in samples collected at different times of the year, while most of the genera were present regardless of the sampling time. Halomonas, Bacillus, and Kushneria species were consistently isolated both from the soil and as endophytes from roots of all three plant species at all collection times. Non-culturable bacterial species were analyzed by Illumina DNA sequencing. The most commonly identified bacteria were from several phyla commonly found in soil or extreme environments: Acidobacteria, Actinobacteria, Bacteroidetes, Chloroflexi, and Gamma- and Delta-Proteobacteria. Isolates were tested for the ability to stimulate growth of alfalfa under saline conditions. This screening led to the identification of one Halomonas and one Bacillus isolate that, when used to inoculate young alfalfa seedlings, stimulate plant growth in the presence of 1 % NaCl, a level that significantly inhibits growth of uninoculated plants. The same bacteria used in the inoculation were recovered from surface sterilized alfalfa roots, indicating the ability of the inoculum to become established as an endophyte. The results with these isolates have exciting promise for enhancing the growth of inoculated alfalfa in salty soil.
Author Address [Kearl, Jennifer; McNary, Caitlyn; Smith, Steven T.; West, Jason; Colton, Emily; Hamson, Michelle; Nielsen, Brent L.] Brigham Young Univ, Dept Microbiol & Mol Biol, Provo, UT 84602 USA; [Lowman, J. Scott; Mei, Chuansheng] Inst Adv Learning & Res, Plant Endophyte Res Ctr, Danville, VA USA; [Aanderud, Zachary T.] Brigham Young Univ, Dept Plant & Wildlife Sci, Provo, UT 84602 USA
Reprint Address Nielsen, BL (corresponding author), Brigham Young Univ, Dept Microbiol & Mol Biol, Provo, UT 84602 USA.
E-mail Address brentnieisen@byu.edu
Funding Agency and Grant Number Roger and Victoria Sant Educational Endowment for a Sustainable Environment; John Topham and Susan Redd Butler BYU Faculty Research Award (The Charles Redd Center for Western Studies); Department of Microbiology and Molecular Biology, Brigham Young University
Funding Text This work was supported by a grant from the Roger and Victoria Sant Educational Endowment for a Sustainable Environment, a John Topham and Susan Redd Butler BYU Faculty Research Award (The Charles Redd Center for Western Studies), and the Department of Microbiology and Molecular Biology, Brigham Young University.
Times Cited 7
Total Times Cited Count (WoS, BCI, and CSCD) 7
Publisher FRONTIERS MEDIA SA
Publisher City LAUSANNE
Publisher Address AVENUE DU TRIBUNAL FEDERAL 34, LAUSANNE, CH-1015, SWITZERLAND
ISSN 1664-302X
29-Character Source Abbreviation FRONT MICROBIOL
ISO Source Abbreviation Front. Microbiol.
Publication Date AUG 14
Year Published 2019
Volume 10
Article Number 1849
Digital Object Identifier (DOI) 10.3389/fmicb.2019.01849
Page Count 11
Web of Science Category Microbiology
Subject Category Microbiology
Document Delivery Number IQ4FO
Unique Article Identifier WOS:000480706700003
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