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Authors Ma, JB; Xiao, XL; Li, L; Maggio, A; Zhang, DY; Mohamad, OAA; Van Oosten, M; Huang, G; Sun, YF; Tian, CY; Yao, YA
Author Full Name Ma, Jinbiao; Xiao, Xinlong; Li, Li; Maggio, Albino; Zhang, Dayong; Mohamad, Osama Abdalla Abdelshafy; Van Oosten, Michael; Huang, Gang; Sun, Yufang; Tian, Changyan; Yao, Yinan
Title Large-scale de novo transcriptome analysis reveals specific gene expression and novel simple sequence repeats markers in salinized roots of the euhalophyte Salicornia europaea
Source ACTA PHYSIOLOGIAE PLANTARUM
Language English
Document Type Article
Author Keywords Abiotic stress; Illumina/Solexa sequencing; Salicornia europaea root; Transcriptome; qRT-PCR
Keywords Plus CONFERRED SALT TOLERANCE; PROLINE ACCUMULATION; ARABIDOPSIS-THALIANA; TRANSGENIC TOBACCO; MOLECULAR-BIOLOGY; NA+/H+ ANTIPORTER; STRESS TOLERANCE; HIGHER-PLANTS; HEAVY-METAL; RNA-SEQ
Abstract Glycophytic plants suffer from severe stress and injury when roots are exposed to high salinity in the rhizosphere. In contrast, the euhalophyte Salicornia europaea grows well at 200 mM NaCl and can withstand up to 1000 mM NaCl in the root zone. Analysis of gene expression profiles and the underlying molecular mechanisms responsible for this tolerance have been largely overlooked. Using the Illumina sequencing platform and the short-reads assembly programme Trinity, we generated a total of 40 and 39 million clean reads and further 140,086 and 122,728 unigenes from the 200 mM NaCl and 0 mM NaCl treated tissues of S. europaea roots, respectively. All unigenes in this study were functionally annotated within context of the COG, GO and KEGG pathways. Unigene functional annotation analysis allowed us to identify hundreds of ion transporters related to homeostasis and osmotic adaptation as well as a variety of proteins related to cation, amino acid, lipid and sugar transport. We found significant enrichment in response to stress including the functional categories of
Author Address [Ma, Jinbiao; Xiao, Xinlong; Li, Li; Mohamad, Osama Abdalla Abdelshafy; Huang, Gang; Sun, Yufang; Tian, Changyan; Yao, Yinan] Chinese Acad Sci, Xinjiang Inst Ecol & Geog, Key Lab Biogeog & Bioresource Arid Land, Urumqi 830011, Peoples R China; [Xiao, Xinlong; Sun, Yufang] Univ Chinese Acad Sci, Beijing 100049, Peoples R China; [Ma, Jinbiao] Shanghai Key Lab Bioenergy Crops, Shanghai, Peoples R China; [Maggio, Albino; Van Oosten, Michael] Univ Naples Federico II, Dept Agr Sci, Via Univ 100, I-80055 Portici, Italy; [Zhang, Dayong] Jiangsu Acad Agr Sci, Inst Agrobiotechnol, Prov Key Lab Agrobiol, Nanjing 210014, Jiangsu, Peoples R China; [Mohamad, Osama Abdalla Abdelshafy] Arish Univ, Inst Post Grad Environm Studies, Dept Environm Sci, North Sinai 45511, Egypt
Reprint Address Tian, CY; Yao, YA (corresponding author), Chinese Acad Sci, Xinjiang Inst Ecol & Geog, Key Lab Biogeog & Bioresource Arid Land, Urumqi 830011, Peoples R China.
E-mail Address tiancy@ms.xjb.ac.cn; yaoya@ms.xjb.ac.cn
ResearcherID Number XIAO, Xinlong/I-6330-2012
ORCID Number ABDELSHAFY MOHAMED, OSAMA ABDALLA/0000-0002-2234-8273
Funding Agency and Grant Number Natural Science Foundation in ChinaNational Natural Science Foundation of China (NSFC) [U1703106]; Youth Innovation Promotion Association, CAS [2016381]; Open Fund of the Shanghai Key Laboratory of Bio-Energy Crops
Funding Text This work was supported by Natural Science Foundation in China (Grant no. U1703106), Youth Innovation Promotion Association, CAS (2016381), and the Open Fund of the Shanghai Key Laboratory of Bio-Energy Crops.
Times Cited 1
Total Times Cited Count (WoS, BCI, and CSCD) 1
Publisher SPRINGER HEIDELBERG
Publisher City HEIDELBERG
Publisher Address TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY
ISSN 0137-5881
29-Character Source Abbreviation ACTA PHYSIOL PLANT
ISO Source Abbreviation Acta Physiol. Plant.
Publication Date AUG
Year Published 2018
Volume 40
Issue 8
Article Number 140
Digital Object Identifier (DOI) 10.1007/s11738-018-2702-z
Page Count 15
Web of Science Category Plant Sciences
Subject Category Plant Sciences
Document Delivery Number GM7IZ
Unique Article Identifier WOS:000438359400001
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