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Authors Lee, SW; Kim, JH; Song, H; Seok, JK; Hong, SS; Boo, YC
Author Full Name Lee, Seok Won; Kim, Jae Heon; Song, Hyerim; Seok, Jin Kyung; Hong, Seong Su; Boo, Yong Chool
Title Luteolin 7-Sulfate Attenuates Melanin Synthesis through Inhibition of CREB- and MITF-Mediated Tyrosinase Expression
Source ANTIOXIDANTS
Language English
Document Type Article
Author Keywords melanin; luteolin 7-sulfate; luteolin; tyrosinase; flavonoid
Keywords Plus P-COUMARIC ACID; CELLULAR MELANOGENESIS; IN-VITRO; FLAVONOIDS; CELLS; RESVERATROL; QUERCETIN; SULFATION; EXERT; CAMP
Abstract Antioxidants with antimelanogenic activity are potentially useful for the attenuation of skin hyperpigmentation disorders. In a previous study, luteolin 7-sulfate isolated from Phyllospadix iwatensis Makino, a marine plant, was shown to inhibit cellular melanin synthesis. The aim of the present study was to examine its action mechanism, focusing on the regulation of tyrosinase (TYR) expression in cells. Cell-based assay was undertaken using murine melanoma B16-F10 cells and primary human epidermal melanocytes (HEMs). Luteolin 7-sulfate showed lower toxicity compared to luteolin in B16-F10 cells. At the non-toxic concentration ranges, luteolin 7-sulfate attenuated melanin synthesis, stimulated by -melanocyte-stimulating hormone or forskolin. Luteolin 7-sulfate attenuated forskolin-induced microphthalmia-associated transcription factor (MITF) and TYR expressions at the mRNA and protein levels in B16-F10 cells. It also attenuated the phosphorylation of cAMP-responsive element binding protein (CREB) stimulated by forskolin. Luteolin 7-sulfate also attenuated melanin synthesis in primary HEMs. This study demonstrates that luteolin 7-sulfate attenuates TYR gene expression through the intervention of a CREB- and MITF-mediated signaling pathway, leading to the decreased melanin synthesis.
Author Address [Lee, Seok Won; Kim, Jae Heon; Song, Hyerim; Seok, Jin Kyung; Boo, Yong Chool] Kyungpook Natl Univ, Sch Med, BK21 Plus KNU Biomed Convergence Program, Dept Mol Med,Cell & Matrix Res Inst, 680 Gukchaebosang Ro, Daegu 41944, South Korea; [Hong, Seong Su] GBSA, Bioctr, Suwon 16229, South Korea
Reprint Address Boo, YC (corresponding author), Kyungpook Natl Univ, Sch Med, BK21 Plus KNU Biomed Convergence Program, Dept Mol Med,Cell & Matrix Res Inst, 680 Gukchaebosang Ro, Daegu 41944, South Korea.
E-mail Address seokwon2891@daum.net; rkrsh@naver.com; happyhyerim@knu.ac.kr; haute87@naver.com; bestgene@gbsa.or.kr; ycboo@knu.ac.kr
ORCID Number Boo, Yong Chool/0000-0001-9895-2066
Funding Agency and Grant Number National Research Foundation of Korea (NRF) - Ministry of Education, Republic of Korea [NRF-2014R1 A1A2053768, NRF-2016R1 D1A1B03932501]
Funding Text This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2014R1 A1A2053768, NRF-2016R1 D1A1B03932501), Republic of Korea.
Times Cited 5
Total Times Cited Count (WoS, BCI, and CSCD) 5
Publisher MDPI
Publisher City BASEL
Publisher Address ST ALBAN-ANLAGE 66, CH-4052 BASEL, SWITZERLAND
29-Character Source Abbreviation ANTIOXIDANTS-BASEL
ISO Source Abbreviation Antioxidants
Publication Date APR
Year Published 2019
Volume 8
Issue 4
Article Number 87
Digital Object Identifier (DOI) 10.3390/antiox8040087
Page Count 11
Web of Science Category Biochemistry & Molecular Biology; Chemistry, Medicinal; Food Science & Technology
Subject Category Biochemistry & Molecular Biology; Pharmacology & Pharmacy; Food Science & Technology
Document Delivery Number HX3MB
Unique Article Identifier WOS:000467294900011
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