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Authors Guidi, L; Brunetti, C; Fini, A; Agati, G; Ferrini, F; Gori, A; Tattini, M
Author Full Name Guidi, Lucia; Brunetti, Cecilia; Fini, Alessio; Agati, Giovanni; Ferrini, Francesco; Gori, Antonella; Tattini, Massimiliano
Title UV radiation promotes flavonoid biosynthesis, while negatively affecting the biosynthesis and the de-epoxidation of xanthophylls: Consequence for photoprotection?
Source ENVIRONMENTAL AND EXPERIMENTAL BOTANY
Language English
Document Type Article
Author Keywords Carotenoids; Chloroplast flavonoids; Excess visible light; Nonphotochemical quenching; Oleaceae; Quercetin; Zeaxanthin
Keywords Plus ULTRAVIOLET-B RADIATION; HIGH SOLAR-RADIATION; ROOT-ZONE SALINITY; LIGUSTRUM-VULGARE; CHLOROPHYLL FLUORESCENCE; VIOLAXANTHIN DEEPOXIDATION; PHOTOSYNTHETIC PIGMENT; ANTIOXIDANT DEFENSE; ENERGY-DISSIPATION; FUNCTIONAL ROLES
Abstract There is evidence that UV radiation may detrimentally affect the biosynthesis of carotenoids, particularly de-epoxided xanthophylls, while strongly promoting phenylpropanoid, particularly flavonoid biosynthesis in a range of taxa. Here we tested the hypothesis that mesophyll flavonoids might protect chloroplasts from UV-induced photo-oxidative damage, by partially compensating for the UV-induced depression of xanthophyll biosynthesis. To test this hypothesis we grew two members of the Oleaceae family, Ligustrum vulgare L. and Phillyrea latifolia L, under either partial shading or fully exposed to sunlight, in the presence or in the absence of UV radiation. The examined species, which display very similar flavonoid composition, largely differ in their ability to limit the transmission of UV and visible light through the leaf and, hence, in the accumulation of flavonoids in mesophyll cells. We conducted measurements of photosynthesis, chlorophyll a fluorescence kinetics, the concentrations of individual carotenoids and phenylpropanoids at the level of whole-leaf, as well as the content of epidermal flavonoids. We also performed multispectral fluorescence micro-imaging to unveil the intra-cellular distribution of flavonoids in mesophyll cells. UV radiation decreased the concentration of carotenoids, particularly of xanthophylls, while greatly promoting the accumulation of flavonoids in palisade parenchyma cells. These effects were much greater in L. vulgare than in P. latifolia. UV radiation significantly inhibited the de-epoxidation of xanthophyll cycle pigments, while enhancing the concentration of luteolin, and particularly of quercetin glycosides. Flavonoids accumulated in the vacuole and the chloroplasts in palisade cells proximal to the adaxial epidermis. We hypothesize that flavonoids might complement the photo-protective functions of xanthophylls in the chloroplasts of mesophyll cells exposed to the greatest doses of UV radiation. However, UV radiation might result in adaxial mesophyll cells being less effective in dissipating the excess of radiant energy, e.g., by decreasing their capacity of thermal dissipation of excess visible light in the chloroplast. (C) 2016 Elsevier B.V. All rights reserved.
Author Address [Guidi, Lucia] Univ Pisa, Dept Agr Food & Environm, I-56124 Pisa, Italy; [Brunetti, Cecilia] Natl Res Council Italy CNR, Trees & Timber Inst, I-50019 Florence, Italy; [Brunetti, Cecilia; Fini, Alessio; Ferrini, Francesco; Gori, Antonella] Univ Florence, Dept Agri Food Prod & Environm Sci, I-50019 Florence, Italy; [Agati, Giovanni] Natl Res Council Italy CNR, Inst Appl Phys Nello Carrara, I-50019 Florence, Italy; [Tattini, Massimiliano] Natl Res Council Italy CNR, Inst Sustainable Plant Protect, I-50019 Florence, Italy
Reprint Address Tattini, M (corresponding author), Natl Res Council Italy CNR, Inst Sustainable Plant Protect, I-50019 Florence, Italy.
E-mail Address massimiliano.tattini@ipsp.cnr.it
ResearcherID Number Cecilia, Brunetti/Q-4724-2018; Tattini, Massimiliano/K-1238-2019; GORI, ANTONELLA/Q-5222-2018
ORCID Number Cecilia, Brunetti/0000-0002-8531-6076; Tattini, Massimiliano/0000-0001-5434-8860; AGATI, GIOVANNI/0000-0003-0855-9389; GUIDI, LUCIA/0000-0002-2472-720X; GORI, ANTONELLA/0000-0002-7304-7526; FINI, ALESSIO/0000-0002-9713-689X
Funding Agency and Grant Number PRIN Project TreeCity (MIUR, Rome, Italy)Ministry of Education, Universities and Research (MIUR)
Funding Text Work in the authors' laboratory was funded partly by the PRIN Project TreeCity (MIUR, Rome, Italy).
Times Cited 19
Total Times Cited Count (WoS, BCI, and CSCD) 20
Publisher PERGAMON-ELSEVIER SCIENCE LTD
Publisher City OXFORD
Publisher Address THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND
ISSN 0098-8472
29-Character Source Abbreviation ENVIRON EXP BOT
ISO Source Abbreviation Environ. Exp. Bot.
Publication Date JUL
Year Published 2016
Volume 127
Beginning Page 14
Ending Page 25
Digital Object Identifier (DOI) 10.1016/j.envexpbot.2016.03.002
Page Count 12
Web of Science Category Plant Sciences; Environmental Sciences
Subject Category Plant Sciences; Environmental Sciences & Ecology
Document Delivery Number DL3EJ
Unique Article Identifier WOS:000375516700002
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