Loading content, please wait..
loading..
Logo
Version 3.21
or
Authors Fan, D; Subramanian, S; Smith, DL
Author Full Name Fan, Di; Subramanian, Sowmyalakshmi; Smith, Donald L.
Title Plant endophytes promote growth and alleviate salt stress in Arabidopsis thaliana
Source SCIENTIFIC REPORTS
Language English
Document Type Article
Keywords Plus BACTERIAL ENDOPHYTES; HYDROGEN-PEROXIDE; OXIDATIVE STRESS; SALINITY; DROUGHT; TOLERANCE; SYSTEM; RHIZOBACTERIA; MAIZE; WATER
Abstract Plant growth promoting rhizobacteria (PGPR) are a functionally diverse group of microbes having immense potential as biostimulants and stress alleviators. Their exploitation in agro-ecosystems as an eco-friendly and cost-effective alternative to traditional chemical inputs may positively affect agricultural productivity and environmental sustainability. The present study describes selected rhizobacteria, from a range of origins, having plant growth promoting potential under controlled conditions. A total of 98 isolates (ectophytic or endophytic) from various crop and uncultivated plants were screened, out of which four endophytes (n, L, K and Y) from Phalaris arundinacea, Solanum dulcamara, Scorzoneroides autumnalis, and Glycine max, respectively, were selected in vitro for their vegetative growth stimulating effects on Arabidopsis thaliana Col-0 seedlings with regard to leaf surface area and shoot fresh weight. A 16S rRNA gene sequencing analysis of the strains indicated that these isolates belong to the genera Pseudomonas, Bacillus, Mucilaginibacter and Rhizobium. Strains were then further tested for their effects on abiotic stress alleviation under both Petri-plate and pot conditions. Results from Petri-dish assay indicated strains L, K and Y alleviated salt stress in Arabidopsis seedlings, while strains K and Y conferred increases in fresh weight and leaf area under osmotic stress. Results from subsequent in vivo trials indicated all the isolates, especially strains L, K and Y, distinctly increased A. thaliana growth under both normal and high salinity conditions, as compared to control plants. The activity of antioxidant enzymes (ascorbate peroxidase, catalase and peroxidase), proline content and total antioxidative capacity also differed in the inoculated A. thaliana plants. Furthermore, a study on spatial distribution of the four strains, using either conventional Petri-plate counts or GFP-tagged bacteria, indicated that all four strains were able to colonize the endosphere of A. thaliana root tissue. Thus, the study revealed that the four selected rhizobacteria are good candidates to be explored as plant growth stimulators, which also possess salt stress mitigating property, partially by regulating osmolytes and antioxidant enzymes. Moreover, the study is the first report of Scorzoneroides autumnalis (fall dandelion) and Solanum dulcamara (bittersweet) associated endophytes with PGP effects.
Author Address [Fan, Di; Subramanian, Sowmyalakshmi; Smith, Donald L.] McGill Univ, Dept Plant Sci, Macdonald Campus,21111 Lakeshore Rd, Ste Anne De Bellevue, PQ H9X 3V9, Canada
Reprint Address Smith, DL (corresponding author), McGill Univ, Dept Plant Sci, Macdonald Campus,21111 Lakeshore Rd, Ste Anne De Bellevue, PQ H9X 3V9, Canada.
E-mail Address donald.smith@mcgill.ca
Funding Agency and Grant Number Natural Sciences and Engineering Research Council (NSERC) of CanadaNatural Sciences and Engineering Research Council of Canada
Funding Text The authors thank the financial support from the Natural Sciences and Engineering Research Council (NSERC) of Canada.
Publisher NATURE PUBLISHING GROUP
Publisher City LONDON
Publisher Address MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND
ISSN 2045-2322
29-Character Source Abbreviation SCI REP-UK
ISO Source Abbreviation Sci Rep
Publication Date JUL 29
Year Published 2020
Volume 10
Issue 1
Article Number 12740
Digital Object Identifier (DOI) 10.1038/s41598-020-69713-5
Page Count 18
Web of Science Category Multidisciplinary Sciences
Subject Category Science & Technology - Other Topics
Document Delivery Number MV5GU
Unique Article Identifier WOS:000556387100006
Plants associated with this reference

LEGAL NOTICES — This website is protected by Copyright © The University of Sussex, 2012, 2013, 2014, 2015, 2016, 2017, 2018, 2019, 2020, 2021. The eHALOPH database is protected by Database Right and Copyright © The University of Sussex and other contributors, 2006, 2012, 2013, 2014, 2015, 2016, 2017, 2018, 2019, 2020, 2021. This database is based on an earlier work by James Aronson.
THIS WEBSITE AND THIS DATABASE ARE PROVIDED ON AN "AS IS" BASIS, AND YOU USE THEM AND RELY ON THEM AT YOUR OWN RISK.

Contact email: halophytes@sussex.ac.uk
Credits – Tim Flowers, Joaquim Santos, Moritz Jahns, Brian Warburton, Peter Reed