Loading content, please wait..
loading..
Logo
Version 3.22
or
Authors Pan, L; Zhang, J; Wang, JZ; Yu, Q; Bai, LY; Dong, LY
Author Full Name Pan, Lang; Zhang, Jian; Wang, Junzhi; Yu, Qin; Bai, Lianyang; Dong, Liyao
Title iTRAQ-based quantitative proteomic analysis reveals proteomic changes in three fenoxaprop-P-ethyl-resistant Beckmannia syzigachne biotypes with differing ACCase mutations
Source JOURNAL OF PROTEOMICS
Language English
Document Type Article
Author Keywords Beckmannia syzigachne Steud; Acetyl-CoA carboxylase (ACCase); Herbicide resistance; Proteomics; Photosynthetic capacity; Fatty acid biosynthesis
Keywords Plus PSI-N SUBUNIT; SALT STRESS; ACETYL-COENZYME; PHOTOSYSTEM-I; HERBICIDE RESISTANCE; ARABIDOPSIS-THALIANA; CARBOXYLASE; MECHANISMS; INHIBITORS; STEUD.
Abstract American sloughgrass (Beckmannia syzigachne Steud.) is a weed widely distributed in wheat fields of China. In recent years, the evolution of herbicide (fenoxaprop-P-ethyl)-resistant populations has decreased the susceptibility of B. syzigachne. This study compared 4 B. syzigachne populations (3 resistant and 1 susceptible) using iTRAQ to characterize fenoxaprop-P-ethyl resistance in B. syzigachne at the proteomic level. Through searching the UniProt database, 3104 protein species were identified from 13,335 unique peptides. Approximately 2834 protein species were assigned to 23 functional classifications provided by the COG database. Among these, 2299 protein species were assigned to 125 predicted pathways. The resistant biotype contained 8 protein species that changed in abundance relative to the susceptible biotype; they were involved in photosynthesis, oxidative phosphorylation, and fatty acid biosynthesis pathways. In contrast to previous studies comparing only 1 resistant and 1 susceptible population, our use of 3 fenoxaprop-resistant B. syzigachne populations with different genetic backgrounds minimized irrelevant differential expression and eliminated false positives. Therefore, we could more confidently link the differentially expressed proteins to herbicide resistance. Proteomic analysis demonstrated that fenoxaprop-P-ethyl resistance is associated with photosynthetic capacity, a connection that might be related to the target-site Mutations in resistant B. syzigachne. This is the first large-scale proteomics study examining herbicide stress responses in different B. syzigachne biotypes. Biological significance: This study has biological relevance because it is the first to employ proteomic analysis for understanding the mechanisms underlying Beckmannia syzigachne herbicide resistance. The plant is a major weed in China and negatively affects crop yield, but has developed considerable resistance to the most common herbicide, fenoxaprop-P-ethyl. Through comparisons of resistant and sensitive biotypes, our study identified multiple proteins (involved in photosynthesis, oxidative phosphorylation, and fatty acid biosynthesis) that are putatively linked to B. syzigachne herbicide response. This large-scale proteomics study, sorely lacking in weed science, contributes valuable data that can be applied to more fine-tuned analyses on the functions of specific proteins in herbicide resistance. (C) 2017 Elsevier B.V. All rights reserved.
Author Address [Pan, Lang; Zhang, Jian; Wang, Junzhi; Dong, Liyao] Nanjing Agr Univ, Coll Plant Protect, Nanjing 210095, Jiangsu, Peoples R China; [Pan, Lang; Zhang, Jian; Dong, Liyao] Nanjing Agr Univ, Minist Educ, Key Lab Integrated Management Crop Dis & Pests, Nanjing 210095, Jiangsu, Peoples R China; [Yu, Qin] Univ Western Australia, Sch Plant Biol, AHRI, Crawley, WA 6009, Australia; [Bai, Lianyang] Hunan Acad Agr Sci, Biotechnol Res Ctr, Changsha 410125, Hunan, Peoples R China
Reprint Address Dong, LY (corresponding author), Nanjing Agr Univ, Coll Plant Protect, Nanjing 210095, Jiangsu, Peoples R China.
E-mail Address dly@njau.edu.cn
ORCID Number Yu, Qin/0000-0001-6379-9444
Funding Agency and Grant Number National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31572021]
Funding Text This research was funded by National Natural Science Foundation of China (31572021). The authors thank Zhang Youming (Jiangdu Plant Protection Station, China) and Zhu Longfen (Wujin Plant Protection Station, China) for providing seeds of B. syzigachne.
Times Cited 1
Total Times Cited Count (WoS, BCI, and CSCD) 2
Publisher ELSEVIER SCIENCE BV
Publisher City AMSTERDAM
Publisher Address PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS
ISSN 1874-3919
29-Character Source Abbreviation J PROTEOMICS
ISO Source Abbreviation J. Proteomics
Publication Date MAY 8
Year Published 2017
Volume 160
Beginning Page 47
Ending Page 54
Digital Object Identifier (DOI) 10.1016/j.jprot.2017.03.018
Page Count 8
Web of Science Category Biochemical Research Methods
Subject Category Biochemistry & Molecular Biology
Document Delivery Number EU7LX
Unique Article Identifier WOS:000401217400006
Plants associated with this reference

LEGAL NOTICES — This website is protected by Copyright © The University of Sussex, 2012, 2013, 2014, 2015, 2016, 2017, 2018, 2019, 2020, 2021, 2022. The eHALOPH database is protected by Database Right and Copyright © The University of Sussex and other contributors, 2006, 2012, 2013, 2014, 2015, 2016, 2017, 2018, 2019, 2020, 2021, 2022. This database is based on an earlier work by James Aronson.
THIS WEBSITE AND THIS DATABASE ARE PROVIDED ON AN "AS IS" BASIS, AND YOU USE THEM AND RELY ON THEM AT YOUR OWN RISK.

Contact email: halophytes@sussex.ac.uk
Credits – Tim Flowers, Joaquim Santos, Moritz Jahns, Brian Warburton, Peter Reed