Loading content, please wait..
loading..
Logo
Version 3.22
or
Authors Menezes, TM; Gaiao, WDC; Lima, LCDS; da Silva, AKB; Lima, LWS; Pereira, AMD; da Silva, LC; da Silva, VL; Franco, ED; Paz, ST; Maia, CS; da Silva, TMS; Maia, MBD
Author Full Name Menezes, Tamires Meira; Cardoso Gaiao, Wyndly Daniel; de Almeida Sousa Lima, Larissa Caroline; Bezerra da Silva, Ana Katarina; Santos Lima, Laisa Wanessa; de Souza Pereira, Aurea Marcela; da Silva, Luciano Clemente; da Silva, Valdir Luna; Franco, Eryvelton de Souza; Paz, Silvania Tavares; Maia, Carina Scanoni; Sarmento da Silva, Tania Maria; de Sousa Maia, Maria Bernadete
Title Evaluation of acute oral toxicity, embryotoxicity and cytotoxicity of the polar fraction of Parkinsonia aculeata aerial parts extract
Source TOXICOLOGY RESEARCH
Language English
Document Type Article
Author Keywords Parkinsonia aculeata; toxicology; diabetes mellitus
Keywords Plus MEDICINAL-PLANTS; AQUEOUS EXTRACT; INSULIN-RESISTANCE; DIABETES-MELLITUS; SUBACUTE TOXICITY; ZEBRAFISH; MODEL; RAT; TOXICOLOGY; SYSTEMS
Abstract Ethnopharmacobotanical information reports that Parkinsonia aculeata infusion is used to control diabetes-related complications and dyslipidemia. However, few studies are reported on the safe use of this species. The aim of this study is to evaluate the acute toxicity, embryotoxicity and cytotoxicity of a polar fraction obtained from hydroethanolic extract of P. aculeata (PfrHEPA). For the acute toxicity test, we considered the Up and Down method which the guidelines are described by the Organization for Economic Cooperation and Development (OECD N. 425). The animals were treated with PfrHEPA (2000 mg/kg) or with distilled water (10 ml/kg) by gavage and observed from Day 1 to14. For embryotoxicity assay, zebrafish embryos were exposed to PfrHEPA (100 mg/L) and toxicity parameters were observed during four consecutive days. The cytotoxicity of PfrHEPA (5, 10, 25, 50, 75 and 100 mu g/ml, respectively) was performed on normal cell lines (mesenchymal stem cells, African green monkey renal cells and mouse pre-adipocytes 3 T3-L1 using the MTT salt reduction assay. In the acute toxicity test, no mortality was observed in mice treated with PfrHEPA (2000 mg/kg), as well as behavioral changes, histopathological abnormalities and hematological and biochemical variables. In the embryotoxicity test, no abnormal changes related to the toxicological parameters were observed in the period of 96 h. Regarding the cytotoxicity assay, PfrHEPA showed no cytotoxic effect on the normal cell lines tested, with an IC50 value > 100 mu g/ml. These results suggest the safe use of P. aculeata, however, more trials are needed for PfrHEPA to be presented as new safe therapeutic proposal for the control of metabolic disorders.
Author Address [Menezes, Tamires Meira; Cardoso Gaiao, Wyndly Daniel; de Almeida Sousa Lima, Larissa Caroline; Bezerra da Silva, Ana Katarina; Santos Lima, Laisa Wanessa; de Souza Pereira, Aurea Marcela; da Silva, Luciano Clemente; da Silva, Valdir Luna; de Sousa Maia, Maria Bernadete] Univ Fed Pernambuco, Dept Physiol & Pharmacol, Recife, PE, Brazil; [Franco, Eryvelton de Souza] Brazilian Univ Ctr, Dept Vet Med, Recife, PE, Brazil; [Paz, Silvania Tavares] Univ Fed Pernambuco, Dept Pathol, Recife, PE, Brazil; [Maia, Carina Scanoni] Univ Fed Pernambuco, Dept Histol & Embryol, Recife, PE, Brazil; [Sarmento da Silva, Tania Maria] Rural Fed Univ Pernambuco, Dept Chem, Recife, PE, Brazil
Reprint Address Maia, MBD (corresponding author), Univ Fed Pernambuco, Ctr Biosci, Dept Physiol & Pharmacol, Lab Pharmacol Bioact Prod, Av Moraes Rego S-N, BR-50670910 Recife, PE, Brazil.
E-mail Address mbsm@ufpe.br
Funding Agency and Grant Number Fundacao de Amparo a Ciencia e Tecnologia do Estado de Pernambuco (FACEPE-Brazil) [IBPG-1434-2.10/15]
Funding Text This work was supported by Fundacao de Amparo a Ciencia e Tecnologia do Estado de Pernambuco (FACEPE-Brazil, Process No. IBPG-1434-2.10/15).
Publisher OXFORD UNIV PRESS
Publisher City OXFORD
Publisher Address GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND
ISSN 2045-452X
29-Character Source Abbreviation TOXICOL RES-UK
ISO Source Abbreviation Toxicol. Res.
Publication Date FEB
Year Published 2020
Volume 9
Issue 1
Beginning Page 19
Ending Page 27
Digital Object Identifier (DOI) 10.1093/toxres/tfz002
Page Count 9
Web of Science Category Toxicology
Subject Category Toxicology
Document Delivery Number MG2NL
Unique Article Identifier WOS:000545872200003
Plants associated with this reference

LEGAL NOTICES — This website is protected by Copyright © The University of Sussex, 2012, 2013, 2014, 2015, 2016, 2017, 2018, 2019, 2020, 2021. The eHALOPH database is protected by Database Right and Copyright © The University of Sussex and other contributors, 2006, 2012, 2013, 2014, 2015, 2016, 2017, 2018, 2019, 2020, 2021. This database is based on an earlier work by James Aronson.
THIS WEBSITE AND THIS DATABASE ARE PROVIDED ON AN "AS IS" BASIS, AND YOU USE THEM AND RELY ON THEM AT YOUR OWN RISK.

Contact email: halophytes@sussex.ac.uk
Credits – Tim Flowers, Joaquim Santos, Moritz Jahns, Brian Warburton, Peter Reed