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Authors Tava, A; Pecetti, L
Author Full Name Tava, Aldo; Pecetti, Luciano
Title Chemical Investigation of Saponins from Twelve Annual Medicago Species and their Bioassay with the Brine Shrimp Artemia salina
Source NATURAL PRODUCT COMMUNICATIONS
Language English
Document Type Article
Author Keywords Saponins; Sapogenins; Medicagenic acid; Hederagenin; Bayogenin; Annual Medicago spp.; Artemia salina; Biossay; Toxicity
Keywords Plus TRITERPENE SAPONINS; AERIAL PARTS; L.; GLYCOSIDES; QUANTIFICATION; TOXICITY; LEAVES; ROOTS
Abstract The saponin and sapogenin composition of the aerial growth of 12 annual Medicago species sampled at full senescence were investigated. Saponins were extracted from the plant material and obtained in a highly pure grade by reverse-phase chromatography, with a yield ranging from 0.38 +/- 0.04% to 1.35 +/- 0.08% dry matter, depending on the species. Sapogenins were then obtained after acid hydrolysis of saponins, and evaluated by GC/FID and GC/MS methods. Different compositions of the aglycone moieties were observed in the 12 Medicago species. Medicagenic acid was the dominant aglycone in M. x blancheana, M. doliata, M. littoralis, M. rotala, M. rugosa, M. scutellata, M. tomato and M. truncatula, bayogenin and hederagenin in M. arabica and M. rigidula, echinocystic acid in M. polymorpha, and soyasapogenol B in M. aculeata. The purified saponin mixtures, characterized by different chemical compositions, were then used in a toxicity test using the brine shrimp Anemia sauna. The most active compounds were the saponins from M. arabica and M rigidula with LD50 values of 10.1 and 4.6 mu g/mL, respectively. A structure-activity relationship for the tested saponin mixtures was observed.
Author Address [Tava, Aldo; Pecetti, Luciano] CRA FLC Ctr Ric Prod Foraggere & Lattiero Caseari, I-26900 Lodi, Italy
Reprint Address Tava, A (reprint author), CRA FLC Ctr Ric Prod Foraggere & Lattiero Caseari, Viale Piacenza 29, I-26900 Lodi, Italy.
E-mail Address aldo.tava@entecra.it
Times Cited 11
Total Times Cited Count (WoS, BCI, and CSCD) 11
Publisher SAGE PUBLICATIONS INC
Publisher City THOUSAND OAKS
Publisher Address 2455 TELLER RD, THOUSAND OAKS, CA 91320 USA
ISSN 1934-578X
29-Character Source Abbreviation NAT PROD COMMUN
ISO Source Abbreviation Nat. Prod. Commun.
Publication Date JUL
Year Published 2012
Volume 7
Issue 7
Beginning Page 837
Ending Page 840
Page Count 4
Web of Science Category Chemistry, Medicinal; Food Science & Technology
Subject Category Pharmacology & Pharmacy; Food Science & Technology
Document Delivery Number 978RW
Unique Article Identifier WOS:000306764200008
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