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| Authors | Tava, A; Pecetti, L |
| Author Full Name | Tava, Aldo; Pecetti, Luciano |
| Title | Chemical Investigation of Saponins from Twelve Annual Medicago Species and their Bioassay with the Brine Shrimp Artemia salina |
| Source | NATURAL PRODUCT COMMUNICATIONS |
| Language | English |
| Document Type | Article |
| Author Keywords | Saponins; Sapogenins; Medicagenic acid; Hederagenin; Bayogenin; Annual Medicago spp.; Artemia salina; Biossay; Toxicity |
| Keywords Plus | TRITERPENE SAPONINS; AERIAL PARTS; L.; GLYCOSIDES; QUANTIFICATION; TOXICITY; LEAVES; ROOTS |
| Abstract | The saponin and sapogenin composition of the aerial growth of 12 annual Medicago species sampled at full senescence were investigated. Saponins were extracted from the plant material and obtained in a highly pure grade by reverse-phase chromatography, with a yield ranging from 0.38 +/- 0.04% to 1.35 +/- 0.08% dry matter, depending on the species. Sapogenins were then obtained after acid hydrolysis of saponins, and evaluated by GC/FID and GC/MS methods. Different compositions of the aglycone moieties were observed in the 12 Medicago species. Medicagenic acid was the dominant aglycone in M. x blancheana, M. doliata, M. littoralis, M. rotala, M. rugosa, M. scutellata, M. tomato and M. truncatula, bayogenin and hederagenin in M. arabica and M. rigidula, echinocystic acid in M. polymorpha, and soyasapogenol B in M. aculeata. The purified saponin mixtures, characterized by different chemical compositions, were then used in a toxicity test using the brine shrimp Anemia sauna. The most active compounds were the saponins from M. arabica and M rigidula with LD50 values of 10.1 and 4.6 mu g/mL, respectively. A structure-activity relationship for the tested saponin mixtures was observed. |
| Author Address | [Tava, Aldo; Pecetti, Luciano] CRA FLC Ctr Ric Prod Foraggere & Lattiero Caseari, I-26900 Lodi, Italy |
| Reprint Address | Tava, A (reprint author), CRA FLC Ctr Ric Prod Foraggere & Lattiero Caseari, Viale Piacenza 29, I-26900 Lodi, Italy. |
| E-mail Address | aldo.tava@entecra.it |
| Times Cited | 11 |
| Total Times Cited Count (WoS, BCI, and CSCD) | 11 |
| Publisher | SAGE PUBLICATIONS INC |
| Publisher City | THOUSAND OAKS |
| Publisher Address | 2455 TELLER RD, THOUSAND OAKS, CA 91320 USA |
| ISSN | 1934-578X |
| 29-Character Source Abbreviation | NAT PROD COMMUN |
| ISO Source Abbreviation | Nat. Prod. Commun. |
| Publication Date | JUL |
| Year Published | 2012 |
| Volume | 7 |
| Issue | 7 |
| Beginning Page | 837 |
| Ending Page | 840 |
| Page Count | 4 |
| Web of Science Category | Chemistry, Medicinal; Food Science & Technology |
| Subject Category | Pharmacology & Pharmacy; Food Science & Technology |
| Document Delivery Number | 978RW |
| Unique Article Identifier | WOS:000306764200008 |
| Plants associated with this reference |