Loading content, please wait..
loading..
Logo
Version 3.21
or
Authors Jena, SN; Verma, S; Nair, KN; Srivastava, AK; Misra, S; Rana, TS
Author Full Name Jena, Satya Narayan; Verma, Sushma; Nair, Kuttan Narayanan; Srivastava, Awadhesh Kumar; Misra, Sujata; Rana, Tikam Singh
Title Genetic diversity and population structure of the mangrove lime (Merope angulata) in India revealed by AFLP and ISSR markers
Source AQUATIC BOTANY
Language English
Document Type Article
Author Keywords AFLP; Genetic diversity; ISSR; Merope angulata; Population genetic structure; Rutaceae
Keywords Plus CONSERVATION GENETICS; CHINA; RAPD; INFERENCE; SOFTWARE; PROGRAMS; SERIES; TREES; FLOW
Abstract The mangrove lime, Merope angulata (Willd.) Swingle (Rutaceae) is a threatened mangrove associate species occurring sporadically in the tidal forests from Papua New Guinea to the east coast of India. It is an underutilized salt tolerant plant related to Citrus. We have applied AFLP and ISSR markers to estimate the extent of genetic diversity among 55 individuals of M. angulata, sampled from two natural populations (Bhitarkanika Wildlife Sanctuary) and one ex situ population (Jharkhali Mangrove Ecological Garden) in India. A combined analysis of both AFLP and ISSR markers revealed low polymorphism (42.93%) and a moderate heterozygosity and Shannon diversity index (H= 0.393, 1=0.571) in the species. The Jaccard genetic distance among 55 accessions ranged from 0.16 to 0.74 (average 0.43). Analysis of population genetic structure using Bayesian method in STRUCTURE software identified two genetic clusters. F-ST statistics and AMOVA results indicated low population differentiation with high gene flow. Haplotype indices and mismatch distributions indicated some evidence of expansion in the two natural populations. Outcrossing test revealed a predominantly out-breeding system with high outcrossing rate. The high gene flow detected among the populations indicates that M. angulata does not face any genetic drift, and hence no local genetic differentiation and population divergence. The highly fragmented state of its habitats warrants adequate measures for maintenance of genetic diversity in this species without further depletion. This is the first report on molecular analysis of genetic diversity and population structure of M. angulata from its native distributional range in India. (C) 2014 Elsevier B.V. All rights reserved.
Author Address [Jena, Satya Narayan] CSIR, Natl Bot Res Inst, Plant Mol Biol & Genet Engn Div, Lucknow 226001, Uttar Pradesh, India; [Verma, Sushma; Nair, Kuttan Narayanan; Srivastava, Awadhesh Kumar; Misra, Sujata; Rana, Tikam Singh] CSIR, Natl Bot Res Inst, Plant Divers Systemat & Herbarium Div, Lucknow 226001, Uttar Pradesh, India
Reprint Address Nair, KN (reprint author), CSIR, Natl Bot Res Inst, Plant Divers Systemat & Herbarium Div, Rana Pratap Marg, Lucknow 226001, Uttar Pradesh, India.
E-mail Address nairnbri@yahoo.co.in
Funding Agency and Grant Number Council of Scientific and Industrial Research (CSIR), New Delhi, IndiaCouncil of Scientific & Industrial Research (CSIR) - India
Funding Text We thank the Director, CSIR-National Botanical Research Institute (CSIR-NBRI) for providing facilities to carry out this work; the Department of Forests and Wildlife, Orissa and the Secretary, Calcutta Wildlife Society, Jharkhali, West Bengal for having granted permission to collect plant samples; and to the officers & staff of Bhitarkanika National Park (BNP), Kendrapara, Orissa, for their help, support and facilities extended to us during our field study in BNP. The financial assistance received from Council of Scientific and Industrial Research (CSIR), New Delhi, India to support this work is gratefully acknowledged.
Times Cited 14
Total Times Cited Count (WoS, BCI, and CSCD) 14
Publisher ELSEVIER
Publisher City AMSTERDAM
Publisher Address RADARWEG 29, 1043 NX AMSTERDAM, NETHERLANDS
ISSN 0304-3770
29-Character Source Abbreviation AQUAT BOT
ISO Source Abbreviation Aquat. Bot.
Publication Date JAN
Year Published 2015
Volume 120
Part Number B
Beginning Page 260
Ending Page 267
Digital Object Identifier (DOI) 10.1016/j.aquabot.2014.09.004
Page Count 8
Web of Science Category Plant Sciences; Marine & Freshwater Biology
Subject Category Plant Sciences; Marine & Freshwater Biology
Document Delivery Number AY7RG
Unique Article Identifier WOS:000347756100016
Plants associated with this reference

LEGAL NOTICES — This website is protected by Copyright © The University of Sussex, 2012, 2013, 2014, 2015, 2016, 2017, 2018, 2019, 2020, 2021. The eHALOPH database is protected by Database Right and Copyright © The University of Sussex and other contributors, 2006, 2012, 2013, 2014, 2015, 2016, 2017, 2018, 2019, 2020, 2021. This database is based on an earlier work by James Aronson.
THIS WEBSITE AND THIS DATABASE ARE PROVIDED ON AN "AS IS" BASIS, AND YOU USE THEM AND RELY ON THEM AT YOUR OWN RISK.

Contact email: halophytes@sussex.ac.uk
Credits – Tim Flowers, Joaquim Santos, Moritz Jahns, Brian Warburton, Peter Reed