Loading content, please wait..
loading..
Logo
Version 3.24
or
Authors Yamamoto, K; Shiwa, Y; Ishige, T; Sakamoto, H; Tanaka, K; Uchino, M; Tanaka, N; Oguri, S; Saitoh, H; Tsushima, S
Author Full Name Yamamoto, Kosuke; Shiwa, Yuh; Ishige, Taichiro; Sakamoto, Hikaru; Tanaka, Keisuke; Uchino, Masataka; Tanaka, Naoto; Oguri, Suguru; Saitoh, Hiromasa; Tsushima, Seiya
Title Bacterial Diversity Associated With the Rhizosphere and Endosphere of Two Halophytes: Glaux maritima and Salicornia europaea
Source FRONTIERS IN MICROBIOLOGY
Language English
Document Type Article
Author Keywords bacterial diversity; Glaux maritime; Salicornia europaea; halophyte; endophytic bacteria; rhizosphere bacteria; 16S rRNA; metagenome
Keywords Plus COMMUNITY STRUCTURE; MARINE BACTERIUM; ROOT MICROBIOTA; SP NOV.; PLANT; GROWTH; SEAWATER; GENE
Abstract Root-associated microbial communities are very important in the adaptation of halophytes to coastal environments. However, little has been reported on microbial community structures related to halophytes, or on comparisons of their compositions among halophytic plant species. Here, we studied the diversity and community structure of both rhizosphere and root endosphere bacteria in two halophytic plants: Glaux maritima and Salicomia europaea. We sampled the rhizosphere, the root endosphere, and bulk control soil samples, and performed bacterial 16S rRNA sequencing using the Illumina MiSeq platform to characterize the bacterial community diversities in the rhizosphere and root endosphere of both halophytes. Among the G. maritima samples, the richness and diversity of bacteria in the rhizosphere were higher than those in the root endosphere but were lower than those of the bulk soil. In contrast for S. europaea, the bulk soil, the rhizosphere, and the root endosphere all had similar bacterial richness and diversity. The number of unique operational taxonomic units within the root endosphere, the rhizosphere, and the bulk soil were 181, 366, and 924 in G. maritima and 126, 416, and 596 in S. europaea, respectively, implying habitat-specific patterns for each halophyte. In total, 35 phyla and 566 genera were identified. The dominant phyla across all samples were Proteobacteria and Bacteroidetes. Actinobacteria was extremely abundant in the root endosphere from G. maritima. Beneficial bacterial genera were enriched in the root endosphere and rhizosphere in both halophytes. Rhizobium, Actinoplanes, and Marinomonas were highly abundant in G. maritima, whereas Sulfurimonas and Coleofasciculus were highly abundant in S. europaea. A principal coordinate analysis demonstrated significant differences in the microbiota composition associated with the plant species and type of sample. These results strongly indicate that there are clear differences in bacterial community structure and diversity between G. maritima and S. europaea. This is the first report to characterize the root microbiome of G. maritima, and to compare the diversity and community structure of rhizosphere and root endosphere bacteria between G. maritima and S. europaea.
Author Address [Yamamoto, Kosuke; Shiwa, Yuh; Uchino, Masataka; Tanaka, Naoto; Saitoh, Hiromasa; Tsushima, Seiya] Tokyo Univ Agr, Fac Life Sci, Dept Mol Microbiol, Tokyo, Japan; [Shiwa, Yuh; Ishige, Taichiro; Tanaka, Keisuke] Tokyo Univ Agr, NODAI Genome Res Ctr, Tokyo, Japan; [Sakamoto, Hikaru; Oguri, Suguru] Tokyo Univ Agr, Dept Northern Biosphere Agr, Fac Bioind, Tokyo, Hokkaido, Japan
Reprint Address Yamamoto, K (reprint author), Tokyo Univ Agr, Fac Life Sci, Dept Mol Microbiol, Tokyo, Japan.
E-mail Address ky200683@nodai.ac.jp
Funding Agency and Grant Number Japan Society for the Promotion of ScienceMinistry of Education, Culture, Sports, Science and Technology, Japan (MEXT)Japan Society for the Promotion of Science [17H07127]; MEXTMinistry of Education, Culture, Sports, Science and Technology, Japan (MEXT) [S1311017]
Funding Text This research was supported by
Times Cited 4
Total Times Cited Count (WoS, BCI, and CSCD) 4
Publisher FRONTIERS MEDIA SA
Publisher City LAUSANNE
Publisher Address AVENUE DU TRIBUNAL FEDERAL 34, LAUSANNE, CH-1015, SWITZERLAND
ISSN 1664-302X
29-Character Source Abbreviation FRONT MICROBIOL
ISO Source Abbreviation Front. Microbiol.
Publication Date NOV 28
Year Published 2018
Volume 9
Article Number 2878
Digital Object Identifier (DOI) 10.3389/fmicb.2018.02878
Page Count 12
Web of Science Category Microbiology
Subject Category Microbiology
Document Delivery Number HC2PH
Unique Article Identifier WOS:000451643900001
Plants associated with this reference

LEGAL NOTICES — This website is protected by Copyright © The University of Sussex, 2012, 2013, 2014, 2015, 2016, 2017, 2018, 2019, 2020, 2021, 2022. The eHALOPH database is protected by Database Right and Copyright © The University of Sussex and other contributors, 2006, 2012, 2013, 2014, 2015, 2016, 2017, 2018, 2019, 2020, 2021, 2022. This database is based on an earlier work by James Aronson.
THIS WEBSITE AND THIS DATABASE ARE PROVIDED ON AN "AS IS" BASIS, AND YOU USE THEM AND RELY ON THEM AT YOUR OWN RISK.

Contact email: halophytes@sussex.ac.uk
Credits – Tim Flowers, Joaquim Santos, Moritz Jahns, Brian Warburton, Peter Reed