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Publication Type J
Authors Zhang, Y. X., Y. Zhang, J. J. Yu, H. Zhang, L. Y. Wang, S. N. Wang, S. Y. Guo, Y. C. Miao, S. X. Chen, Y. Li and S. J. Dai
Title NaCl-responsive ROS scavenging and energy supply in alkaligrass callus revealed from proteomic analysis
Source Bmc Genomics
Author Keywords Salinity response ROS scavenging Energy supply Osmotic homeostasis Callus Halophyte alkaligrass Proteomics halophyte puccinellia-tenuiflora programmed cell-death somatic embryogenesis medicago-truncatula salinity tolerance nitric-oxide salt stress mechanisms proline embryo
Abstract Background: Salinity has obvious effects on plant growth and crop productivity. The salinity-responsive mechanisms have been well-studied in differentiated organs (e.g., leaves, roots and stems), but not in unorganized cells such as callus. High-throughput quantitative proteomics approaches have been used to investigate callus development, somatic embryogenesis, organogenesis, and stress response in numbers of plant species. However, they have not been applied to callus from monocotyledonous halophyte alkaligrass (Puccinellia tenuifora). Results: The alkaligrass callus growth, viability and membrane integrity were perturbed by 50 mM and 150 mM NaCl treatments. Callus cells accumulated the proline, soluble sugar and glycine betaine for the maintenance of osmotic homeostasis. Importantly, the activities of ROS scavenging enzymes (e.g., SOD, APX, POD, GPX, MDHAR and GR) and antioxidants (e.g., ASA, DHA and GSH) were induced by salinity. The abundance patterns of 55 saltresponsive proteins indicate that salt signal transduction, cytoskeleton, ROS scavenging, energy supply, gene expression, protein synthesis and processing, as well as other basic metabolic processes were altered in callus to cope with the stress. Conclusions: The undifferentiated callus exhibited unique salinity-responsive mechanisms for ROS scavenging and energy supply. Activation of the POD pathway and AsA-GSH cycle was universal in callus and differentiated organs, but salinity-induced SOD pathway and salinity-reduced CAT pathway in callus were different from those in leaves and roots. To cope with salinity, callus mainly relied on glycolysis, but not the TCA cycle, for energy supply.
Author Address [Zhang, Yongxue; Zhang, Yue; Yu, Juanjuan; Wang, Liyue; Wang, Sining; Li, Ying] Northeast Forestry Univ, Key Lab Saline Alkali Vegetat Ecol Restorat, Minist Educ, Coll Life Sci, Harbin 150040, Peoples R China. [Zhang, Yongxue; Zhang, Heng; Dai, Shaojun] Shanghai Normal Univ, Coll Life Sci, Dev Ctr Plant Germplasm Resources, Shanghai 200234, Peoples R China. [Yu, Juanjuan] Henan Normal Univ, Coll Life Sci, Xinxiang 453007, Henan, Peoples R China. [Guo, Siyi; Miao, Yuchen] Henan Univ, Inst Plant Stress Biol, Dept Biol, State Key Lab Cotton Biol, Kaifeng 455000, Peoples R China. [Chen, Sixue] Univ Florida, Interdisciplinary Ctr Biotechnol Res, Plant Mol & Cellular Biol Program, Dept Biol,Genet Inst, Gainesville, FL 32610 USA. Li, Y (reprint author), Northeast Forestry Univ, Key Lab Saline Alkali Vegetat Ecol Restorat, Minist Educ, Coll Life Sci, Harbin 150040, Peoples R China.; Dai, SJ (reprint author), Shanghai Normal Univ, Coll Life Sci, Dev Ctr Plant Germplasm Resources, Shanghai 200234, Peoples R China. ly7966@nefu.edu.cn; daishaojun@hotmail.com
ISSN 1471-2164
ISBN 1471-2164
29-Character Source Abbreviation BMC Genomics
Publication Date Dec
Year Published 2019
Volume 20
Issue 1
Digital Object Identifier (DOI) 10.1186/s12864-019-6325-6
Unique Article Identifier WOS:000508020600003
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