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Authors Espasandin, FD; Calzadilla, PI; Maiale, SJ; Ruiz, OA; Sansberro, PA
Author Full Name Espasandin, Fabiana D.; Calzadilla, Pablo I.; Maiale, Santiago J.; Ruiz, Oscar A.; Sansberro, Pedro A.
Title Overexpression of the Arginine Decarboxylase Gene Improves Tolerance to Salt Stress in Lotus tenuis Plants
Source JOURNAL OF PLANT GROWTH REGULATION
Language English
Document Type Article
Author Keywords Putrescine; Abscisic acid; NCED; Salt tolerance
Keywords Plus POLYAMINE METABOLISM; LIPID-PEROXIDATION; COLD-ACCLIMATION; OXIDATIVE DAMAGE; K+ TRANSPORT; PUTRESCINE; ACCUMULATION; ARABIDOPSIS; MECHANISMS; SALINITY
Abstract To analyse the putative effects of putrescine on the plant response to salinity, micropropagated Lotus tenuis plants from wild-type and transgenic lines harbouring the pRD29A::oat arginine decarboxylase (ADC, EC 4.1.1.19) construct were subjected to a gradual increase in salinity that was applied gradually by means of sodium chloride irrigation (from 0 to 0.3 mol L-1) every 5 days up to the maximum concentration. At the end of the experiment, the transgenic lines were healthier than the wild-type plants and displayed a smaller reduction in shoot biomass and a slight increase in root growth in response to stress. The overexpression of ADC increased osmotic adjustment (5.8-fold) via the release of proline. The salinity treatment doubled the potassium uptake by roots from transgenic ADC stressed plants with a concomitant decrease in the accumulation of sodium, balancing the Na+/K+ ratio. Analysis of gene expression, enzymatic activities and hormone metabolism suggests a crosstalk between polyamines and abscisic acid in response to salinity via modulation of the abscisic acid biosynthesis-related enzyme 9-cis-epoxycarotenoid dioxygenase (EC 1.13.11.51) at the transcriptional level.
Author Address [Espasandin, Fabiana D.; Sansberro, Pedro A.] Univ Nacl Nordeste, Lab Biotecnol Aplicada & Genom Func, Fac Ciencias Agrarias, Inst Botan Nordeste IBONE CONICET, Sargento Cabral 2131,CC 209,W3402BKG, Corrientes, Argentina; [Calzadilla, Pablo I.; Maiale, Santiago J.; Ruiz, Oscar A.] IIB INTECh CONICET, Unidad Biotecnol 1, CC 164,B7130IWA, Chascomus, Argentina
Reprint Address Sansberro, PA (reprint author), Univ Nacl Nordeste, Lab Biotecnol Aplicada & Genom Func, Fac Ciencias Agrarias, Inst Botan Nordeste IBONE CONICET, Sargento Cabral 2131,CC 209,W3402BKG, Corrientes, Argentina.
E-mail Address sansber@agr.unne.edu.ar
ResearcherID Number Sansberro, Pedro/AAG-4588-2019
ORCID Number Sansberro, Pedro/0000-0002-6540-3666; maiale, santiago/0000-0002-8943-7238
Funding Agency and Grant Number Agencia Nacional de Promocion Cientifica y TecnicaANPCyT [PICTO 2011-0213, PICT 2014-1246, PICT 2014-3718]; Secretaria General de Ciencia y Tecnica-Universidad Nacional del Nordeste [PI A001/14]; CONICETConsejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET) [PIP 0980]; CONICETConsejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET)
Funding Text This work was supported by grants from Agencia Nacional de Promocion Cientifica y Tecnica (PICTO 2011-0213, PICT 2014-1246, PICT 2014-3718), Secretaria General de Ciencia y Tecnica-Universidad Nacional del Nordeste (PI A001/14) and from CONICET (PIP 0980). We extend our deep appreciation to anonymous reviewers for their critical comments. F. Espasandin, S. Maiale, O. Ruiz and P. Sansberro are members of the Research Council of Argentina (CONICET). P. Calzadilla received a CONICET scholarship.
Times Cited 4
Total Times Cited Count (WoS, BCI, and CSCD) 4
Publisher SPRINGER
Publisher City NEW YORK
Publisher Address 233 SPRING ST, NEW YORK, NY 10013 USA
ISSN 0721-7595
29-Character Source Abbreviation J PLANT GROWTH REGUL
ISO Source Abbreviation J. Plant Growth Regul.
Publication Date MAR
Year Published 2018
Volume 37
Issue 1
Beginning Page 156
Ending Page 165
Digital Object Identifier (DOI) 10.1007/s00344-017-9713-7
Page Count 10
Web of Science Category Plant Sciences
Subject Category Plant Sciences
Document Delivery Number FY5FF
Unique Article Identifier WOS:000426851300012
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