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Authors Rejii, M; Mahdhi, M; Dominguez-Nunez, JA; Mars, M
Author Full Name Rejii, Mokhtar; Mahdhi, Mosbah; Alfonso Dominguez-Nunez, Jose; Mars, Mohamed
Title The phenotypic, phylogenetic and symbiotic characterization of rhizobia nodulating Lotus sp in Tunisian arid soils
Source ANNALS OF MICROBIOLOGY
Language English
Document Type Article
Author Keywords Lotus sp.; Rhizobia; 16S rRNA gene sequence; Tunisia
Keywords Plus GENETIC DIVERSITY; SP-NOV.; PHASEOLUS-VULGARIS; BACTERIA; LEGUMES; ACACIA; GENUS; POPULATIONS; TOLERANCE; NODULES
Abstract Fifteen bacterial isolates, representatives of different 16S rRNA-RFLP genomogroups which were isolated from root nodules of Lotus creticus and L. pusillus growing in the arid areas of Tunisia were characterized by phenotypic features and 16S rDNA sequences. Phenotypically, all isolates are fast growers with the ability to grow at a pH between 5.5 and 9. Most of the tested isolates tolerate NaCl concentrations from 1.39 to 3.48 %. Phylogenetically, the studied isolates are affiliated into the genera: Sinorhizobium (5 strains), Rhizobium (2 strains), and Mesorhizobium (4 strains). The 16S rDNA sequences of Tunisian Lotus sp. nodule isolates: LAC7511, LAC733, and Mesorhizobium alhagi (Alhagi sparsifolia symbiont) shared 100 % identical nucleotides similar to the 16S rDNA sequences of LAC831, LAC814 and Mesorhizobium temperatum CCNWSX0012-2 (Astragalus adsurgens symbiont). Non-nodulating bacteria, considered as endophytes of Lotus sp. nodules, were also found in our studies and they were classified into the genera: Phyllobacterium (2 strains), Starkeya (1 strain) and Pseudomonas (1 strain). Except for these four endophytic Lotus sp. bacteria, all other strains under investigation induce nodules on Lotus sp., but they differ in the number of induced root nodules and the effectiveness of atmospheric nitrogen fixation. The Sinorhizobium sp., Mesohizobium sp. and Lotus sp. nodule isolates, forming the most effective symbiosis with the plant host, are potential candidates for inoculants in revegetation programs.
Author Address [Rejii, Mokhtar; Mahdhi, Mosbah; Mars, Mohamed] Gabes Univ, Fac Sci, Res Unit Biodivers & Valorizat Arid Areas Bioress, Erriadh Zrig 6072, Gabes, Tunisia; [Alfonso Dominguez-Nunez, Jose] Univ Politecn Madrid, ETSI Montes, E-28040 Madrid, Spain; [Rejii, Mokhtar] Gabes Univ, Fac Sci, Zirig 6072, Gabes, Tunisia
Reprint Address Rejii, M (reprint author), Gabes Univ, Fac Sci, Zirig 6072, Gabes, Tunisia.
E-mail Address rejili_mokhtar@yahoo.fr
ResearcherID Number mosbah, MAHDHI/I-1253-2019
ORCID Number mosbah, MAHDHI/0000-0003-3102-2141
Funding Agency and Grant Number Spanish Agency for International cooperation and Development (AECID)
Funding Text This research work was kindly supported by grants from Spanish Agency for International cooperation and Development (AECID) in the frame of the Tunisia-Spain collaboration project
Times Cited 3
Total Times Cited Count (WoS, BCI, and CSCD) 3
Publisher SPRINGER
Publisher City NEW YORK
Publisher Address 233 SPRING ST, NEW YORK, NY 10013 USA
ISSN 1590-4261
29-Character Source Abbreviation ANN MICROBIOL
ISO Source Abbreviation Ann. Microbiol.
Publication Date MAR
Year Published 2014
Volume 64
Issue 1
Beginning Page 355
Ending Page 362
Digital Object Identifier (DOI) 10.1007/s13213-013-0670-5
Page Count 8
Web of Science Category Biotechnology & Applied Microbiology; Microbiology
Subject Category Biotechnology & Applied Microbiology; Microbiology
Document Delivery Number AB2UT
Unique Article Identifier WOS:000331648300037
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