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Authors Rejili, M; Lorite, MJ; Mahdhi, M; Pinilla, JS; Ferchichi, A; Mars, M
Author Full Name Rejili, Mokhtar; Jose Lorite, Maria; Mahdhi, Mosbah; Sanjuan Pinilla, Juan; Ferchichi, Ali; Mars, Mohamed
Title Genetic diversity of rhizobial populations recovered from three Lotus species cultivated in the infra-arid Tunisian soils
Source PROGRESS IN NATURAL SCIENCE-MATERIALS INTERNATIONAL
Language English
Document Type Article
Author Keywords Lotus sp.; rep-PCR fingerprinting; PCR-RFLP 16S rRNA; Rhizobia; Arid habitat
Keywords Plus NODULATE PHASEOLUS-VULGARIS; POLYMERASE-CHAIN-REACTION; 16S RIBOSOMAL-RNA; GENOTYPIC DIVERSITY; REPETITIVE SEQUENCES; RETAMA-RAETAM; ROOT-NODULES; BACTERIA; L.; STRAINS
Abstract Eighty-three bacterial strains isolated from root nodules of Lotus creticus, L. pusillus, and L. arabicus grown in infra-arid Tunisian soils were characterized using a polyphasic approach including phenotypic analysis, rep-PCR and PCR-RFLP analyses of the 16S rRNA gene. Phenotypically, all isolates are fast growers the majority of which grow at a pH of between 5.5 and 9. Most of the tested isolates tolerate NaCl concentrations from 1.39% to 3.48%. By rep-PCR. fingerprinting, the genomic similarity varied from 30% to 98%. All tested isolates were clustered into 32 rep-PCR clusters at the similarity level of 80%. The genomic divergence of strains revealed by rep/PCR analysis appeared to be very important since a molecular polymorphism delimiting symbionts for each species of Lotus was identified. With the high-resolution of rep-PCR profiles of the isolates obtained using Pearson's/UPGMA analysis, the isolates were resolved into 60 different profiling groups to undergo 16S ARDRA analyses. The analysis of all restriction fragments from each strain based on the UPGMA algorithm from the combined patterns showed that Lotus isolates are very diverse and that they were affiliated to Sinorhizobium, Rhizobium, and Mesorhizobium genera. (C) 2009 National Natural Science Foundation of China and Chinese Academy of Sciences. Published by Elsevier Limited and Science in China Press. All rights reserved.
Author Address [Rejili, Mokhtar; Ferchichi, Ali] Arid Area Inst IRA, Arid & Oases Cropping Lab, Medenine 4119, Tunisia; [Jose Lorite, Maria; Sanjuan Pinilla, Juan] CSIC, Expt Stn Zaidin, Soil Microbiol & Symbiot Syst Dept, Granada, Spain; [Rejili, Mokhtar; Mahdhi, Mosbah; Mars, Mohamed] Fac Sci Gabes, Lab Plant Biotechnol Appl Crop Improvement, Zrig 6072, Gabes, Tunisia
Reprint Address Rejili, M (reprint author), Arid Area Inst IRA, Arid & Oases Cropping Lab, Medenine 4119, Tunisia.
E-mail Address rejili_mokhtar@yahoo.fr
ResearcherID Number mosbah, MAHDHI/I-1253-2019; Lorite, Maria J/L-1696-2014
ORCID Number mosbah, MAHDHI/0000-0003-3102-2141; Lorite, Maria J/0000-0003-2911-2439
Funding Agency and Grant Number AECI-Spain project [A/9631/07]; Ministry of High Education and Research Development-Tunisia
Funding Text This work was supported by AECI-Spain project (No. A/9631/07) and partly supported by the Ministry of High Education and Research Development-Tunisia. We thank MAHDHI Mosbah and LORITE Maria Jose for their technical help and constructive suggestions. We are very grateful to Juan Sanjuan Pinilla for his constructive suggestions and supervision on the work.
Times Cited 14
Total Times Cited Count (WoS, BCI, and CSCD) 14
Publisher ELSEVIER SCIENCE INC
Publisher City NEW YORK
Publisher Address STE 800, 230 PARK AVE, NEW YORK, NY 10169 USA
ISSN 1002-0071
29-Character Source Abbreviation PROG NAT SCI-MATER
ISO Source Abbreviation Prog. Nat. Sci.
Publication Date SEP 10
Year Published 2009
Volume 19
Issue 9
Beginning Page 1079
Ending Page 1087
Digital Object Identifier (DOI) 10.1016/j.pnsc.2009.02.003
Page Count 9
Web of Science Category Materials Science, Multidisciplinary; Multidisciplinary Sciences
Subject Category Materials Science; Science & Technology - Other Topics
Document Delivery Number 481HR
Unique Article Identifier WOS:000268798900007
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