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Authors Dabbous, A; Ben Saad, R; Brini, F; Farhat-Khemekhem, A; Zorrig, W; Abdely, C; Ben Hamed, K
Author Full Name Dabbous, Amira; Ben Saad, Rania; Brini, Faical; Farhat-Khemekhem, Ameny; Zorrig, Walid; Abdely, Chedly; Ben Hamed, Karim
Title Over-expression of a subunit E1 of a vacuolar H+-ATPase gene (Lm VHA-E1) cloned from the halophyte Lobularia maritima improves the tolerance of Arabidopsis thaliana to salt and osmotic stresses
Source ENVIRONMENTAL AND EXPERIMENTAL BOTANY
Language English
Document Type Article
Author Keywords Arabidopsis thaliana; Lobularia maritima; Osmotic stress; Salinity; Transgenic plants; Vacuolar H+-ATPase subunit E1
Keywords Plus SALINITY TOLERANCE; AGROBACTERIUM-TUMEFACIENS; V-ATPASE; OVEREXPRESSION; TRANSFORMATION; QUANTITATION; HOMEOSTASIS; ADAPTATION; RESPONSES; DROUGHT
Abstract In order to understand the molecular basis of vacuolar H+-ATPase subunits and reveal their possible role in salt and drought tolerance in plant species, a vacuolar H+-ATPase subunit E1 gene (LmVHA-E1) was isolated from the halophyte Lobularia maritima and over-expressed in the glycophyte Arabidopsis thaliana. Q-RT-PCR demonstrated that the expression of this gene was induced in L maritima in response to salt stress and PEG-induced osmotic stress. The overexpression of LmVHA-E1 in Arabidopsis thaliana conferred high salt and drought tolerance to transgenic plants. Transgenic Arabidopsis plants showed higher seedling survival rates and better antioxidant activities under salt and drought stress. The analysis of ion content and osmotic potentials indicated that under high salinity the transgenic plants compartmentalized more Na+ and showed enhanced osmotic adjustment in the leaves compared with wild-type plants. Accordingly, the higher levels of expression of different stress related genes such as AtNHX1 AtP5CS, AtCAT, AtSOD, AtPOD and AtLEA, indicated higher levels of activities in sodium sequestration into vacuoles, in osmotic regulation and in ROS scavenging of transgenic plants. (C) 2017 Elsevier B.V. All rights reserved.
Author Address [Dabbous, Amira; Zorrig, Walid; Abdely, Chedly; Ben Hamed, Karim] Ctr Biotechnol Borj Cedria, Lab Extremophile Plants, POB 901, Hammam Lif 2050, Tunisia; [Ben Saad, Rania; Brini, Faical] Univ Sfax, Biotechnol & Plant Improvement Lab, Ctr Biotechnol Sfax, BP 1177, Sfax 3018, Tunisia; [Farhat-Khemekhem, Ameny] Univ Sfax, Ctr Biotechnol Sfax, Lab Microorganisms & Biomol, BP 1177, Sfax 3018, Tunisia
Reprint Address Ben Hamed, K (reprint author), Ctr Biotechnol Borj Cedria, Lab Extremophile Plants, POB 901, Hammam Lif 2050, Tunisia.
E-mail Address kbenhamed@yahoo.fr
ResearcherID Number , karim/AAA-9671-2019; ZORRIG, Walid/J-3428-2013
ORCID Number ZORRIG, Walid/0000-0001-6607-9151
Funding Agency and Grant Number Tunisian Ministry of Higher Education and Scientific Research [LR15CBBC02]
Funding Text This study was supported by the Tunisian Ministry of Higher Education and Scientific Research (LR15CBBC02).
Times Cited 6
Total Times Cited Count (WoS, BCI, and CSCD) 8
Publisher PERGAMON-ELSEVIER SCIENCE LTD
Publisher City OXFORD
Publisher Address THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND
ISSN 0098-8472
29-Character Source Abbreviation ENVIRON EXP BOT
ISO Source Abbreviation Environ. Exp. Bot.
Publication Date MAY
Year Published 2017
Volume 137
Beginning Page 128
Ending Page 141
Digital Object Identifier (DOI) 10.1016/j.envexpbot.2017.01.013
Page Count 14
Web of Science Category Plant Sciences; Environmental Sciences
Subject Category Plant Sciences; Environmental Sciences & Ecology
Document Delivery Number ER4ER
Unique Article Identifier WOS:000398752400012
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