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Authors Yang, RW; Zhou, YH; Zhang, Y; Zheng, YL; Ding, CB
Author Full Name Yang, RW; Zhou, YH; Zhang, Y; Zheng, YL; Ding, CB
Title The genetic diversity among Leymus species based on random amplified microsatellite polymorphism (RAMP)
Source GENETIC RESOURCES AND CROP EVOLUTION
Language English
Document Type Article
Author Keywords cluster analysis; genetic diversity; Leymus; RAMP; relationships
Keywords Plus BARLEY
Abstract The genetic diversity and similarities among 40 accessions of Leymus Hochst., distributed in 19 species and 1 subspecies, were analyzed by using random amplified microsatellite polymorphism (RAMP) markers. Of the 120 RAMP primer combinations tested, 24 (20%) produced polymorphic and clear bands. A total of 192 bands were amplified by 24 primer combinations, among which 179 (93.23%) bands were found to be polymorphic. Three to thirteen polymorphic bands were amplified by each primer combination, with an average of 7.64 bands. The data of 192 RAMP bands were used to generate Jaccard's similarity coefficients and to construct a dendrogram by means of UPGMA in the NTSYS-pc computer program. The genetic similarity coefficients ranged from 0.10 to 0.73 with the mean of 0.34. The results showed as follows: (1) Distinct genetic differences were present among the different species; (2) The different accessions in a species were clustered together, respectively, which had larger genetic similarities and closer relations; (3) The species with similar morphological characters and the species from the same areas or neighboring geographical regions were clustered together; (4) RAMP results are basically comparable with those obtained from studies on morphology. It is a useful method for analysis of the genetic diversity and similarities in Leymus.
Author Address Sichuan Agr Univ, Triticeae Res Inst, Dujiangyan 611830, Sichuan, Peoples R China
Reprint Address Zhou, YH (reprint author), Sichuan Agr Univ, Triticeae Res Inst, Dujiangyan 611830, Sichuan, Peoples R China.
E-mail Address zhouyh@sicau.edu.cn
Times Cited 7
Total Times Cited Count (WoS, BCI, and CSCD) 13
Publisher SPRINGER
Publisher City DORDRECHT
Publisher Address VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS
ISSN 0925-9864
29-Character Source Abbreviation GENET RESOUR CROP EV
ISO Source Abbreviation Genet. Resour. Crop Evol.
Publication Date FEB
Year Published 2006
Volume 53
Issue 1
Beginning Page 139
Ending Page 144
Digital Object Identifier (DOI) 10.1007/s10722-004-1933-6
Page Count 6
Web of Science Category Agronomy; Plant Sciences
Subject Category Agriculture; Plant Sciences
Document Delivery Number 006DD
Unique Article Identifier WOS:000234874500015
Plants associated with this reference

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