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| Authors | Aoki, S; Kondo, T; Prevost, D; Nakata, S; Kajita, T; Ito, M |
| Author Full Name | Aoki, Seishiro; Kondo, Tetsuya; Prevost, Danielle; Nakata, Sayuri; Kajita, Tadashi; Ito, Motomi |
| Title | Genotypic and phenotypic diversity of rhizobia isolated from Lathyrus japonicus indigenous to Japan |
| Source | SYSTEMATIC AND APPLIED MICROBIOLOGY |
| Language | English |
| Document Type | Article |
| Author Keywords | Lathyrus japonicus; Rhizobium leguminorasum; Rhizobium pisi; Dispersing plants; Horizontal gene transfer; nod genes |
| Keywords Plus | LEGUMINOSARUM-BIOVAR-VICIAE; POPULATION GENETIC-STRUCTURE; COMMON NODULATION GENES; RIBOSOMAL-RNA GENE; SP-NOV; PHYLOGENETIC INFERENCE; SUBSTRATE COMPOSITION; MOLECULAR DIVERSITY; EMENDED DESCRIPTION; INVASIVE LEGUME |
| Abstract | Sixty-one rhizobial strains from Lathyrus japonicus nodules growing on the seashore in Japan were characterized and compared to two strains from Canada. The PCR-based method was used to identify test strains with novel taxonomic markers that were designed to discriminate between all known Lathyrus rhizobia. Three genomic groups (I,II, and III) were finally identified using RAPD, RFLP, and phylogenetic analyses. Strains in genomic group I (related to Rhizobium leguminosarum) were divided into two subgroups (Ia and Ib) and subgroup la was related to biovar viciae. Strains in subgroup lb, which were all isolated from Japanese sea pea, belonged to a distinct group from other rhizobial groups in the recA phylogeny and PCR-based grouping, and were more tolerant to salt than the isolate from an inland legume. Test strains in genomic groups II and III belonged to a single clade with the reference strains of R. pisi, R. etli, and R. phaseoli in the 16S rRNA phylogeny. The PCR-based method and phylogenetic analysis of recA revealed that genomic group II was related to R. pisi. The analyses also showed that genomic group III harbored a mixed chromosomal sequence of different genomic groups, suggesting a recent horizontal gene transfer between diverse rhizobia. Although two Canadian strains belonged to subgroup Ia, molecular and physiological analyses showed the divergence between Canadian and Japanese strains. Phylogenetic analysis of nod genes divided the rhizobial strains into several groups that reflected the host range of rhizobia. Symbiosis between dispersing legumes and rhizobia at seashore is discussed. (C) 2010 Elsevier GmbH. All rights reserved. |
| Author Address | [Aoki, Seishiro; Ito, Motomi] Univ Tokyo, Grad Sch Arts & Sci, Dept Gen Syst Studies, Meguro Ku, Tokyo 1538902, Japan; [Kondo, Tetsuya] Hokkaido Univ, Res Fac Agr, Kita Ku, Sapporo, Hokkaido 0608589, Japan; [Prevost, Danielle] Agr & Agri Food Canada, Soils & Crops Res & Dev Ctr, Quebec City, PQ G1V 2J3, Canada; [Nakata, Sayuri; Kajita, Tadashi] Chiba Univ, Fac Sci, Dept Biol, Inage Ku, Chiba 2638522, Japan |
| Reprint Address | Aoki, S (reprint author), Univ Tokyo, Grad Sch Arts & Sci, Dept Gen Syst Studies, Meguro Ku, 3-8-1 Komaba, Tokyo 1538902, Japan. |
| E-mail Address | saoki@bio.c.u-tokyo.ac.jp |
| ResearcherID Number | Kondo, Tetsuya/A-4310-2012 |
| Funding Agency and Grant Number | Ministry of Education, Culture, Sports, Science, and Technology of JapanMinistry of Education, Culture, Sports, Science and Technology, Japan (MEXT) |
| Funding Text | We are grateful to Dr. Shigeto Dobata for his invaluable comments. We thank to Ishikari-hama Seaside Plants Conservation Center, Ishikari, Hokkaido, for giving us the seeds of L japonicus. This work was supported by the Grand-in-Aid for Scientific Research (C) and Scientific Research on Priority Areas 'Comparative Genomics' from the Ministry of Education, Culture, Sports, Science, and Technology of Japan. |
| Times Cited | 17 |
| Total Times Cited Count (WoS, BCI, and CSCD) | 17 |
| Publisher | ELSEVIER GMBH, URBAN & FISCHER VERLAG |
| Publisher City | JENA |
| Publisher Address | OFFICE JENA, P O BOX 100537, 07705 JENA, GERMANY |
| ISSN | 0723-2020 |
| 29-Character Source Abbreviation | SYST APPL MICROBIOL |
| ISO Source Abbreviation | Syst. Appl. Microbiol. |
| Publication Date | NOV |
| Year Published | 2010 |
| Volume | 33 |
| Issue | 7 |
| Beginning Page | 383 |
| Ending Page | 397 |
| Digital Object Identifier (DOI) | 10.1016/j.syapm.2010.07.001 |
| Page Count | 15 |
| Web of Science Category | Biotechnology & Applied Microbiology; Microbiology |
| Subject Category | Biotechnology & Applied Microbiology; Microbiology |
| Document Delivery Number | 708MI |
| Unique Article Identifier | WOS:000286365500004 |
| Plants associated with this reference |