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Authors Aoki, S; Kondo, T; Prevost, D; Nakata, S; Kajita, T; Ito, M
Author Full Name Aoki, Seishiro; Kondo, Tetsuya; Prevost, Danielle; Nakata, Sayuri; Kajita, Tadashi; Ito, Motomi
Title Genotypic and phenotypic diversity of rhizobia isolated from Lathyrus japonicus indigenous to Japan
Source SYSTEMATIC AND APPLIED MICROBIOLOGY
Language English
Document Type Article
Author Keywords Lathyrus japonicus; Rhizobium leguminorasum; Rhizobium pisi; Dispersing plants; Horizontal gene transfer; nod genes
Keywords Plus LEGUMINOSARUM-BIOVAR-VICIAE; POPULATION GENETIC-STRUCTURE; COMMON NODULATION GENES; RIBOSOMAL-RNA GENE; SP-NOV; PHYLOGENETIC INFERENCE; SUBSTRATE COMPOSITION; MOLECULAR DIVERSITY; EMENDED DESCRIPTION; INVASIVE LEGUME
Abstract Sixty-one rhizobial strains from Lathyrus japonicus nodules growing on the seashore in Japan were characterized and compared to two strains from Canada. The PCR-based method was used to identify test strains with novel taxonomic markers that were designed to discriminate between all known Lathyrus rhizobia. Three genomic groups (I,II, and III) were finally identified using RAPD, RFLP, and phylogenetic analyses. Strains in genomic group I (related to Rhizobium leguminosarum) were divided into two subgroups (Ia and Ib) and subgroup la was related to biovar viciae. Strains in subgroup lb, which were all isolated from Japanese sea pea, belonged to a distinct group from other rhizobial groups in the recA phylogeny and PCR-based grouping, and were more tolerant to salt than the isolate from an inland legume. Test strains in genomic groups II and III belonged to a single clade with the reference strains of R. pisi, R. etli, and R. phaseoli in the 16S rRNA phylogeny. The PCR-based method and phylogenetic analysis of recA revealed that genomic group II was related to R. pisi. The analyses also showed that genomic group III harbored a mixed chromosomal sequence of different genomic groups, suggesting a recent horizontal gene transfer between diverse rhizobia. Although two Canadian strains belonged to subgroup Ia, molecular and physiological analyses showed the divergence between Canadian and Japanese strains. Phylogenetic analysis of nod genes divided the rhizobial strains into several groups that reflected the host range of rhizobia. Symbiosis between dispersing legumes and rhizobia at seashore is discussed. (C) 2010 Elsevier GmbH. All rights reserved.
Author Address [Aoki, Seishiro; Ito, Motomi] Univ Tokyo, Grad Sch Arts & Sci, Dept Gen Syst Studies, Meguro Ku, Tokyo 1538902, Japan; [Kondo, Tetsuya] Hokkaido Univ, Res Fac Agr, Kita Ku, Sapporo, Hokkaido 0608589, Japan; [Prevost, Danielle] Agr & Agri Food Canada, Soils & Crops Res & Dev Ctr, Quebec City, PQ G1V 2J3, Canada; [Nakata, Sayuri; Kajita, Tadashi] Chiba Univ, Fac Sci, Dept Biol, Inage Ku, Chiba 2638522, Japan
Reprint Address Aoki, S (reprint author), Univ Tokyo, Grad Sch Arts & Sci, Dept Gen Syst Studies, Meguro Ku, 3-8-1 Komaba, Tokyo 1538902, Japan.
E-mail Address saoki@bio.c.u-tokyo.ac.jp
ResearcherID Number Kondo, Tetsuya/A-4310-2012
Funding Agency and Grant Number Ministry of Education, Culture, Sports, Science, and Technology of JapanMinistry of Education, Culture, Sports, Science and Technology, Japan (MEXT)
Funding Text We are grateful to Dr. Shigeto Dobata for his invaluable comments. We thank to Ishikari-hama Seaside Plants Conservation Center, Ishikari, Hokkaido, for giving us the seeds of L japonicus. This work was supported by the Grand-in-Aid for Scientific Research (C) and Scientific Research on Priority Areas 'Comparative Genomics' from the Ministry of Education, Culture, Sports, Science, and Technology of Japan.
Times Cited 17
Total Times Cited Count (WoS, BCI, and CSCD) 17
Publisher ELSEVIER GMBH, URBAN & FISCHER VERLAG
Publisher City JENA
Publisher Address OFFICE JENA, P O BOX 100537, 07705 JENA, GERMANY
ISSN 0723-2020
29-Character Source Abbreviation SYST APPL MICROBIOL
ISO Source Abbreviation Syst. Appl. Microbiol.
Publication Date NOV
Year Published 2010
Volume 33
Issue 7
Beginning Page 383
Ending Page 397
Digital Object Identifier (DOI) 10.1016/j.syapm.2010.07.001
Page Count 15
Web of Science Category Biotechnology & Applied Microbiology; Microbiology
Subject Category Biotechnology & Applied Microbiology; Microbiology
Document Delivery Number 708MI
Unique Article Identifier WOS:000286365500004
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