Loading content, please wait..
loading..
Logo
Version 3.20
or
Authors Taniguchi, T; Imada, S; Acharya, K; Iwanaga, F; Yamanaka, N
Author Full Name Taniguchi, Takeshi; Imada, Shogo; Acharya, Kumud; Iwanaga, Fumiko; Yamanaka, Norikazu
Title Effect of soil salinity and nutrient levels on the community structure of the root-associated bacteria of the facultative halophyte, Tamarix ramosissima, in southwestern United States
Source JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY
Language English
Document Type Article
Author Keywords arbuscular mycorrhizal fungi; calcium; phosphorus; Proteobacteria; sodium; Tamarix ramosissima; terminal restriction fragment length polymorphism analysis
Keywords Plus FRAGMENT-LENGTH-POLYMORPHISM; GROWTH-PROMOTING BACTERIA; SP NOV.; MICROBIAL DIVERSITY; DEGRADING BACTERIA; MYCORRHIZAL FUNGI; COASTAL SOIL; SALT STRESS; RHIZOSPHERE; SALTCEDAR
Abstract Tamarix ramosissima is a tree species that is highly resistant to salt and drought. The Tamarix species survives in a broad range of environmental salt levels, and invades major river systems in southwestern United States. It may affect root-associated bacteria (RB) by increasing soil salts and nutrients. The effects of RB on host plants may vary even under saline conditions, and the relationship may be important for T. ramosissima. However, to the best of our knowledge, there have been no reports relating to T. ramosissima RB and its association with salinity and nutrient levels. In this study, we have examined this association and the effect of arbuscular mycorrhizal colonization of T. ramosissima on RB because a previous study has reported that colonization of arbuscular mycorrhizal fungi affected the rhizobacterial community (Marschner et al., 2001). T. ramosissima roots were collected from five locations with varying soil salinity and nutrient levels. RB community structures were examined by terminal restriction fragment (T-RF) length polymorphism, cloning, and sequencing analyses. The results suggest that RB richness, or the diversity of T. ramosissima, have significant negative relationships with electrical conductivity (EC), sodium concentration (Na), and the colonization of arbuscular mycorrhizal fungi, but have a significant positive relationship with phosphorus in the soil. However, at each T-RF level, positive correlations between the emergence of some T-RFs and EC or Na were observed. These results indicate that high salinity decreased the to-tal number of RB species, but some saline-tolerant RB species multiplied with increasing salinity levels. The ordination scores of nonmetric multidimensional scale analysis of RB community composition show significant relationships with water content, calcium concentration, available phosphorus, and total nitrogen. These results indicate that the RB diversity and community composition of T. ramosissima are affected by soil salinity and nutrient levels. Sequence analysis detected one Bacteroidetes and eight Proteobacteria species. Most 16S rRNA gene sequences had high similarities with the bacteria isolated from saline conditions, indicating that at least a portion of the RB species observed in T. ramosissima was halotolerant.
Author Address [Taniguchi, Takeshi; Imada, Shogo; Iwanaga, Fumiko; Yamanaka, Norikazu] Tottori Univ, Arid Land Res Ctr, Tottori 6800001, Japan; [Acharya, Kumud] Desert Res Inst, Div Hydrol Sci, Las Vegas, NV 89119 USA
Reprint Address Taniguchi, T (reprint author), Tottori Univ, Arid Land Res Ctr, 1390 Hamasaka, Tottori 6800001, Japan.
E-mail Address takeshi@alrc.tottori-u.ac.jp
ResearcherID Number Taniguchi, Takeshi/C-9364-2019
Funding Agency and Grant Number JSPS Research Fellowships for Young ScientistsMinistry of Education, Culture, Sports, Science and Technology, Japan (MEXT)Japan Society for the Promotion of Science [21-5699]; Global COE program, Global Center of Excellence for Dryland Science
Funding Text This study was partially supported by JSPS Research Fellowships for Young Scientists (No. 21-5699) and the Global COE program, Global Center of Excellence for Dryland Science.
Times Cited 4
Total Times Cited Count (WoS, BCI, and CSCD) 4
Publisher MICROBIOL RES FOUNDATION
Publisher City TOKYO
Publisher Address JAPAN ACAD SOC CENTER BLDG 4-16 YAYOI 2-CHOME, TOKYO, 113-0032, JAPAN
ISSN 0022-1260
29-Character Source Abbreviation J GEN APPL MICROBIOL
ISO Source Abbreviation J. Gen. Appl. Microbiol.
Year Published 2015
Volume 61
Issue 5
Beginning Page 193
Ending Page 202
Digital Object Identifier (DOI) 10.2323/jgam.61.193
Page Count 10
Web of Science Category Biotechnology & Applied Microbiology; Microbiology
Subject Category Biotechnology & Applied Microbiology; Microbiology
Document Delivery Number CX1QS
Unique Article Identifier WOS:000365471300007
Plants associated with this reference

LEGAL NOTICES — This website is protected by Copyright © The University of Sussex, 2012, 2013, 2014, 2015, 2016, 2017, 2018, 2019. The eHALOPH database is protected by Database Right and Copyright © The University of Sussex and other contributors, 2006, 2012, 2013, 2014, 2015, 2016, 2017, 2018, 2019. This database is based on an earlier work by James Aronson.
THIS WEBSITE AND THIS DATABASE ARE PROVIDED ON AN "AS IS" BASIS, AND YOU USE THEM AND RELY ON THEM AT YOUR OWN RISK.

Contact email: halophytes@sussex.ac.uk
Credits – Tim Flowers, Joaquim Santos, Moritz Jahns, Brian Warburton, Peter Reed