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Authors Meng, DL; Yu, XH; Ma, LY; Hu, J; Liang, YL; Liu, XD; Yin, HQ; Liu, HW; He, XJ; Li, DQ
Author Full Name Meng, Delong; Yu, Xianghua; Ma, Liyuan; Hu, Jin; Liang, Yili; Liu, Xueduan; Yin, Huaqun; Liu, Hongwei; He, Xiaojia; Li, Diqiang
Title Transcriptomic Response of Chinese Yew (Taxus chinensis) to Cold Stress
Source FRONTIERS IN PLANT SCIENCE
Language English
Document Type Article
Author Keywords Taxus chinensis; transcriptome; DE isoforms; cold stress; ROS; cold response
Keywords Plus ROOT HYDRAULIC CONDUCTIVITY; ORYZA-SATIVA L.; RNA-SEQ; PURINE METABOLITE; GENE-EXPRESSION; WATER-UPTAKE; TOLERANCE; PATHWAYS; ROLES; SALT
Abstract Taxus chinensis is a rare and endangered shrub, highly sensitive to temperature changes and widely known for its potential in cancer treatment. How gene expression of T chinensis responds to low temperature is still unknown. To investigate cold response of the genus Taxus, we obtained the transcriptome profiles of T. chinensis grown under normal and low temperature (cold stress, 0 degrees C) conditions using Illumina Miseq sequencing. A transcriptome including 83,963 transcripts and 62,654 genes were assembled from 4.16 Gb of reads data. Comparative transcriptomic analysis identified 2,025 differently expressed (DE) isoforms at p < 0.05, of which 1,437 were up-regulated by cold stress and 588 were down-regulated. Annotation of DE isoforms indicated that transcription factors (TFs) in the MAPK signaling pathway and TF families of NAC, WRKY, bZIP, MYB, and ERF were transcriptionally activated. This might have been caused by the accumulation of secondary messengers, such as reactive oxygen species (ROS) and Ca2+. While accumulation of ROS will have caused damages to cells, our results indicated that to adapt to low temperatures T. chinensis employed a series of mechanisms to minimize these damages. The mechanisms included: (i) cold-enhanced expression of ROS deoxidant systems, such as peroxidase and phospholipid hydroperoxide glutathione peroxidase, to remove ROS. This was further confirmed by analyses showing increased activity of POD, SOD, and CAT under cold stress. (ii) Activation of starch and sucrose metabolism, thiamine metabolism, and purine metabolism by cold-stress to produce metabolites which either protect cell organelles or lower the ROS content in cells. These processes are regulated by ROS signaling, as the
Author Address [Meng, Delong; Yu, Xianghua; Ma, Liyuan; Hu, Jin; Liang, Yili; Liu, Xueduan; Yin, Huaqun; Liu, Hongwei] Cent S Univ, Sch Minerals Proc & Bioengn, Changsha, Hunan, Peoples R China; [Meng, Delong; Yu, Xianghua; Ma, Liyuan; Hu, Jin; Liang, Yili; Liu, Xueduan; Yin, Huaqun; Liu, Hongwei] Cent S Univ, Key Lab Biomet, Minist Educ, Changsha, Hunan, Peoples R China; [Meng, Delong] Univ Coll Dublin, Sch Biol & Environm Sci, Dublin, Ireland; [He, Xiaojia] Adm Ctr Chinas Agenda 21, Beijing, Peoples R China; [Li, Diqiang] Chinese Acad Forestry, Inst Forest Ecol Environm & Protect, State Forestry Adm, Key Lab Forest Ecol & Environm, Beijing, Peoples R China
Reprint Address Yu, XH (reprint author), Cent S Univ, Sch Minerals Proc & Bioengn, Changsha, Hunan, Peoples R China.; Yu, XH (reprint author), Cent S Univ, Key Lab Biomet, Minist Educ, Changsha, Hunan, Peoples R China.
E-mail Address weall2000@126.com
ResearcherID Number Meng, Delong/S-2665-2016
ORCID Number Xianghua, Yu/0000-0002-4096-5523
Funding Agency and Grant Number National Key Technology R&D Program in ChinaNational Key Technology R&D Program [2013BAC09B00-7]; Chinese Postdoctoral Science FoundationChina Postdoctoral Science Foundation; Postdoctoral research funding plan in Hunan Province; Central South University
Funding Text This work was supported by the following grants: the National Key Technology R&D Program (2013BAC09B00-7) in China, Chinese Postdoctoral Science Foundation for DM and HE and Postdoctoral research funding plan in Hunan Province and Central South University for DM and HL. We would also like to thank Mr. Zixia Huang (PhD at University College Dublin, Ireland) for transcriptomic analyses. Great thanks to Dr. Wieland Fricke (University College Dublin, Ireland) for language corrections.
Times Cited 9
Total Times Cited Count (WoS, BCI, and CSCD) 9
Publisher FRONTIERS MEDIA SA
Publisher City LAUSANNE
Publisher Address AVENUE DU TRIBUNAL FEDERAL 34, LAUSANNE, CH-1015, SWITZERLAND
ISSN 1664-462X
29-Character Source Abbreviation FRONT PLANT SCI
ISO Source Abbreviation Front. Plant Sci.
Publication Date APR 28
Year Published 2017
Volume 8
Article Number 468
Digital Object Identifier (DOI) 10.3389/fpls.2017.00468
Page Count 11
Web of Science Category Plant Sciences
Subject Category Plant Sciences
Document Delivery Number ET4VI
Unique Article Identifier WOS:000400282800001
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