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Authors Burch, AY; Finkel, OM; Cho, JK; Belkin, S; Lindow, SE
Author Full Name Burch, Adrien Y.; Finkel, Omri M.; Cho, Juliana K.; Belkin, Shimshon; Lindow, Steven E.
Title Diverse Microhabitats Experienced by Halomonas variabilis on Salt-Secreting Leaves
Source APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Language English
Document Type Article
Keywords Plus BETAINE TRANSPORT-SYSTEM; ESCHERICHIA-COLI; MICROBIAL COMMUNITIES; PSEUDOMONAS-PUTIDA; DESERT TREE; BACTERIA; PHYLLOSPHERE; PROU; CONSTRUCTION; EXPRESSION
Abstract The leaf surfaces of the salt-excreting tree Tamarix aphylla harbor a wide diversity of halophilic microorganisms, including Halomonas sp., but little is known of the factors that shape community composition in this extreme habitat. We isolated a strain of Halomonas variabilis from the leaf surface of T. aphylla and used it to determine the heterogeneity of salt concentrations experienced by bacteria in this environment. This halophilic strain was transformed with a proU::gfp reporter gene fusion, the fluorescence of which was responsive to NaCl concentrations up to 200 g liter(-1). These bioreporting cells were applied to T. aphylla leaves and were subsequently recovered from dew droplets adhering to the leaf surface. Although cells from within a given dew droplet exhibited similar green fluorescent protein fluorescence, the fluorescence intensity varied between droplets and was correlated with the salt concentration measured in each drop. Growth of H. variabilis was observed in all droplets, regardless of the salt concentration. However, cells found in desiccated microniches between dew drops were low in abundance and generally dead. Other bacteria recovered from T. aphylla displayed higher desiccation tolerance than H. variabilis, both in culture and on inoculated plants, despite having lower osmotic tolerance. Thus, the Tamarix leaf surface can be described as a salty desert with occasional oases where water droplets form under humid conditions. While halotolerant bacteria such as Halomonas grow in high concentrations of salt in such wet microniches, other organisms are better suited to survive desiccation in sites that are not wetted.
Author Address [Burch, Adrien Y.; Cho, Juliana K.; Lindow, Steven E.] Univ Calif Berkeley, Dept Plant & Microbial Biol, Berkeley, CA 94720 USA; [Finkel, Omri M.; Belkin, Shimshon] Hebrew Univ Jerusalem, Inst Life Sci, IL-91904 Jerusalem, Israel
Reprint Address Lindow, SE (reprint author), Univ Calif Berkeley, Dept Plant & Microbial Biol, Berkeley, CA 94720 USA.
E-mail Address icelab@berkeley.edu
ResearcherID Number Belkin, Shimshon/L-3469-2019
ORCID Number Belkin, Shimshon/0000-0001-8283-1673
Funding Agency and Grant Number U.S.-Israel Binational Science FoundationUS-Israel Binational Science Foundation
Funding Text Research was supported by the U.S.-Israel Binational Science Foundation.
Times Cited 9
Total Times Cited Count (WoS, BCI, and CSCD) 9
Publisher AMER SOC MICROBIOLOGY
Publisher City WASHINGTON
Publisher Address 1752 N ST NW, WASHINGTON, DC 20036-2904 USA
ISSN 0099-2240
29-Character Source Abbreviation APPL ENVIRON MICROB
ISO Source Abbreviation Appl. Environ. Microbiol.
Publication Date FEB
Year Published 2013
Volume 79
Issue 3
Beginning Page 845
Ending Page 852
Digital Object Identifier (DOI) 10.1128/AEM.02791-12
Page Count 8
Web of Science Category Biotechnology & Applied Microbiology; Microbiology
Subject Category Biotechnology & Applied Microbiology; Microbiology
Document Delivery Number 076WL
Unique Article Identifier WOS:000313989700011
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