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Publication Type J
Authors Meng, L; Li, SS; Guo, JY; Guo, Q; Mao, PC; Tian, XX
Author Full Name Meng, Lin; Li, Shanshan; Guo, Jingya; Guo, Qiang; Mao, Peichun; Tian, Xiaoxia
Title Molecular cloning and functional characterisation of an H+-pyrophosphatase from &ITIris lactea&IT
Source SCIENTIFIC REPORTS
Language English
Document Type Article
Keywords Plus SALT TOLERANCE; INORGANIC PYROPHOSPHATASE; THELLUNGIELLA-HALOPHILA; TONOPLAST VESICLES; NA+/H+ ANTIPORT; ION-TRANSPORT; SUAEDA-SALSA; PPASE GENE; VACUOLAR; OVEREXPRESSION
Abstract Tonoplast H+-pyrophosphatases (VPs) mediate vacuolar Na+ sequestration, a process important for salt tolerance of plants. The function of VP in the highly drought- and salt-tolerant perennial Iris lactea under salt stress is unclear. Here, we isolated IIVP from l. lactea and investigated its function in transgenic tobacco. IIVP was found to comprise 771 amino acid residues and showed 88% similarity with Arabidopsis AtVP1. IIVP was mainly expressed in shoots and was up-regulated by salt stress. Overexpression of IIVP enhanced growth of transgenic tobacco plants compared with wild-type (WT) plants exposed to salt stress. Transgenic plants accumulated higher quantities of Na+ and K+ in leaves, stems, and roots under salt stress, which caused higher leaf relative water content and decreased cell membrane damage compared with WT plants. Overall, IIVP encoding a tonoplast H+-pyrophosphatase can reduce Na+ toxicity in plant cells through increased sequestration of ions into vacuoles by enhanced H+-pyrophosphatase activity.
Author Address [Meng, Lin; Li, Shanshan; Guo, Jingya; Guo, Qiang; Mao, Peichun; Tian, Xiaoxia] Beijing Acad Agr & Forestry Sci, Beijing Res & Dev Ctr Grass & Environm, Beijing 100097, Peoples R China
Reprint Address Meng, L (reprint author), Beijing Acad Agr & Forestry Sci, Beijing Res & Dev Ctr Grass & Environm, Beijing 100097, Peoples R China.
E-mail Address menglin9599@sina.com
Funding Agency and Grant Number Scientific Innovation Ability Construction Project of the Beijing Academy of Agriculture and Forestry Sciences [KJCX20140103]; Beijing Natural Science Foundation [6142007]
Funding Text This research was financially supported by the Beijing Natural Science Foundation (grant no. 6142007) and the Scientific Innovation Ability Construction Project (grant no. KJCX20140103) of the Beijing Academy of Agriculture and Forestry Sciences.
Cited Reference Count 46
Times Cited 1
Total Times Cited Count (WoS, BCI, and CSCD) 1
Publisher NATURE PUBLISHING GROUP
Publisher City LONDON
Publisher Address MACMILLAN BUILDING, 4 CRINAN ST, LONDON N1 9XW, ENGLAND
ISSN 2045-2322
29-Character Source Abbreviation SCI REP-UK
ISO Source Abbreviation Sci Rep
Publication Date DEC 19
Year Published 2017
Volume 7
Article Number 17779
Digital Object Identifier (DOI) 10.1038/s41598-017-18032-3
Page Count 10
Web of Science Category Multidisciplinary Sciences
Subject Category Science & Technology - Other Topics
Document Delivery Number FQ4WQ
Unique Article Identifier WOS:000418359600029
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