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Publication Type J
Authors Gu, CS; Liu, LQ; Deng, YM; Zhu, XD; Lu, XQ; Huang, SZ
Author Full Name Gu, Chun-Sun; Liu, Liang-Qin; Deng, Yan-Ming; Zhu, Xu-Dong; Lu, Xiao-Qing; Huang, Su-Zhen
Title Validation of reference genes for RT-qPCR normalization in Iris. lactea var. chinensis leaves under different experimental conditions
Source SCIENTIA HORTICULTURAE
Language English
Document Type Article
Author Keywords Iris. lactea var. chinensis; Reference genes; RT-qPCR; Abiotic stress
Keywords Plus POLYMERASE-CHAIN-REACTION; REAL-TIME PCR; EXPRESSION NORMALIZATION; GOSSYPIUM-HIRSUTUM; ABIOTIC STRESS; SELECTION; IDENTIFICATION; ACCUMULATION; TOLERANCE
Abstract Iris. lactea var. chinensis (I. lactea var. chinensis) is highly tolerant of Cu, Pb, drought, NaCl and cold. To study the molecular. mechanism of L lactea var. chinensis under these stresses, it is necessary to select reliable reference genes for RT-qPCR study. In the present study, the expression stability of nine candidate reference genes, namely, beta-tubulin (TUBLIN6), ACTIN11, translation initiation factor 4A-1 (EIF4A1), cytosolic phosphoglycerate kinase (PGK),TIP41-like family protein (TIP41), glyceraldehyde-3-phosphate dehydrogenase (GAP), phosphatase 2A (PP2Acs), cyclophilin (CYP) and ubiquitin (UBQ), was assessed in L lactea var. chinensis plants subjected to five abiotic stresses using GeNorm and NormFinder. A combination of GAP, ACTIN11, TIP41, EIF4A1 and PGK was identified as appropriate genes for normalization under PEG stress, whereas the combination of UBQ PGK, TIP41 and PP2Acs was the most suitable under NaCl stress. PP2Acs, ACTIN11 and UBQ exhibited the most stable expression under Pb stress. For Cu-treated leaves, CYP, GAP and TIP41 were the most stably expressed, while GAP, PP2Acs and TIP41 for cold-treated leaves. Generally, the analyses found that TIP41, CYP, PGK, GAP and PP2Acs were the most stable genes under five abiotic stress conditions. These results would contribute the more accurate and widespread use of RT-qPCR in I. lactea var. chinensis gene analysis. (C) 2014 Elsevier B.V. All rights reserved.
Author Address [Gu, Chun-Sun; Lu, Xiao-Qing; Huang, Su-Zhen] Jiangsu Prov & Chinese Acad Sci, Inst Bot, Nanjing 210014, Jiangsu, Peoples R China; [Liu, Liang-Qin; Zhu, Xu-Dong] Nanjing Agr Univ, Coll Hort, Nanjing 210014, Jiangsu, Peoples R China; [Deng, Yan-Ming] Jiangsu Acad Agr Sci, Inst Agrobiotechnol, Nanjing 210014, Jiangsu, Peoples R China
Reprint Address Lu, XQ (reprint author), Jiangsu Prov & Chinese Acad Sci, Inst Bot, Nanjing 210014, Jiangsu, Peoples R China.
E-mail Address hsz1959@163.com
Funding Agency and Grant Number JiangSu Provincial Key LAB Foundation For Plant EX SITU Conservation [201201]; National Natural Science Foundation of China [31301807]; Science Foundation of Jiangsu [BK20130734]
Funding Text The study was supported by the JiangSu Provincial Key LAB Foundation For Plant EX SITU Conservation (no. 201201), National Natural Science Foundation of China (31301807), Science Foundation of Jiangsu (BK20130734)
Cited Reference Count 43
Times Cited 8
Total Times Cited Count (WoS, BCI, and CSCD) 9
Publisher ELSEVIER SCIENCE BV
Publisher City AMSTERDAM
Publisher Address PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS
ISSN 0304-4238
29-Character Source Abbreviation SCI HORTIC-AMSTERDAM
ISO Source Abbreviation Sci. Hortic.
Publication Date AUG 15
Year Published 2014
Volume 175
Beginning Page 144
Ending Page 149
Digital Object Identifier (DOI) 10.1016/j.scienta.2014.06.011
Page Count 6
Web of Science Category Horticulture
Subject Category Agriculture
Document Delivery Number AN0YD
Unique Article Identifier WOS:000340309000017
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