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Publication Type J
Authors Zhang, H; Zheng, JX; Su, HX; Xia, KF; Jian, HG; Zhang, M
Author Full Name Zhang, Hui; Zheng, Jiexuan; Su, Huaxiang; Xia, Kuaifei; Jian, Huguang; Zhang, Mei
Title Molecular Cloning and Functional Characterization of the Dehydrin (IpDHN) Gene From Ipomoea pes-caprae
Source FRONTIERS IN PLANT SCIENCE
Language English
Document Type Article
Author Keywords Ipomoea pes-caprae L.; dehydrin; salt; drought; promoter
Keywords Plus SALT-STRESS TOLERANCE; SAUSSUREA-INVOLUCRATA KAR.; SALINITY TOLERANCE; DROUGHT TOLERANCE; OSMOTIC-STRESS; LEA PROTEINS; K-SEGMENTS; ARABIDOPSIS; EXPRESSION; PROTECTION
Abstract Dehydrin (DHN) genes can be rapidly induced to offset water deficit stresses in plants. Here, we reported on a dehydrin gene (IpDHN) related to salt tolerance isolated from Ipomoea pes-caprae L. (Convolvulaceae). The IpDHN protein shares a relatively high homology with Arabidopsis dehydrin ERD14 (At1g76180). IpDHN was shown to have a cytoplasmic localization pattern. Quantitative RT-PCR analyses indicated that IpDHN was differentially expressed in most organs of I. pes-caprae plants, and its expression level increased after salt, osmotic stress, oxidative stress, cold stress and ABA treatments. Analysis of the 974-bp promoter of IpDHN identified distinct cis-acting regulatory elements, including an MYB binding site (MBS), ABRE (ABA responding)-elements, Skn-1 motif, and TC-rich repeats. The induced expression of IpDHN in Escherichia coli indicated that IpDHN might be involved in salt, drought, osmotic, and oxidative stresses. We also generated transgenic Arabidopsis lines that over-expressed IpDHN. The transgenic Arabidopsis plants showed a significant enhancement in tolerance to salt/drought stresses, as well as less accumulation of hydrogen peroxide (H2O2) and the superoxide radical (O-2(-)), accompanied by increasing activity of the antioxidant enzyme system in vivo. Under osmotic stresses, the overexpression of IpDHN in Arabidopsis can elevate the expression of ROS-related and stress-responsive genes and can improve the ROS-scavenging ability. Our results indicated that IpDHN is involved in cellular responses to salt and drought through a series of pleiotropic effects that are likely involved in ROS scavenging and therefore influence the physiological processes of microorganisms and plants exposed to many abiotic stresses.
Author Address [Zhang, Hui; Zheng, Jiexuan; Su, Huaxiang; Xia, Kuaifei; Zhang, Mei] Chinese Acad Sci, Key Lab South China Agr Plant Mol Anal & Genet Im, South China Bot Garden, Guangzhou, Guangdong, Peoples R China; [Zhang, Hui; Zheng, Jiexuan; Su, Huaxiang] Univ Chinese Acad Sci, Coll Life Sci, Beijing, Peoples R China; [Xia, Kuaifei; Jian, Huguang; Zhang, Mei] Chinese Acad Sci, Key Lab Appl Bot, South China Bot Garden, Guangzhou, Guangdong, Peoples R China
Reprint Address Zhang, M (reprint author), Chinese Acad Sci, Key Lab South China Agr Plant Mol Anal & Genet Im, South China Bot Garden, Guangzhou, Guangdong, Peoples R China.; Zhang, M (reprint author), Chinese Acad Sci, Key Lab Appl Bot, South China Bot Garden, Guangzhou, Guangdong, Peoples R China.
E-mail Address zhangmei@scbg.ac.cn
Funding Agency and Grant Number 'Strategic Priority Research Program' of the Chinese Academy of Sciences [XDA13020500]; National Key Research and Development Program of China [2016YFC1403002]; Twelfth Five-Year National S&T Projects for Rural Areas [2015BAL04B04]
Funding Text This research was supported by grants from the 'Strategic Priority Research Program' of the Chinese Academy of Sciences (XDA13020500), the National Key Research and Development Program of China (2016YFC1403002), and
Cited Reference Count 70
Publisher FRONTIERS MEDIA SA
Publisher City LAUSANNE
Publisher Address AVENUE DU TRIBUNAL FEDERAL 34, LAUSANNE, CH-1015, SWITZERLAND
ISSN 1664-462X
29-Character Source Abbreviation FRONT PLANT SCI
ISO Source Abbreviation Front. Plant Sci.
Publication Date OCT 11
Year Published 2018
Volume 9
Article Number 1454
Digital Object Identifier (DOI) 10.3389/fpls.2018.01454
Page Count 20
Web of Science Category Plant Sciences
Subject Category Plant Sciences
Document Delivery Number GW5OO
Unique Article Identifier WOS:000446985400001
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