School of Life Sciences

photo of Steve Sweet

Dr Steve Sweet

Post:Senior Research Fellow (Genome Damage and Stability)
Location:GENOME CENTRE G3.05
Email:S.M.Sweet@sussex.ac.uk

Telephone numbers
Internal:7414
UK:01273 877414
International:+44 1273 877414

Research expertise:
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Biography

Post-doctoral research

[2009-2011] Postdoctoral Researcher, Northwestern University, IL, USA. Professor Neil Kelleher.

I applied dual label stable-isotope labeling (SILAC) to differentiate both new and old histones and new and old methylation. This allowed us to observe the re-establishment of methylation that occurs after DNA replication or co-transcriptionally. Histone PTMs have been suggested to be epigenetic in nature: the ‘inheritance’ of methylation status after cell division is therefore highly relevant. Interestingly, our findings for H3K79 methylation suggested a certain amount of positional ‘scrambling’ occurs.

An additional project analyzed global histone alterations that occur as a result of the multiple myeloma t(4;14) translocation and MMSET methyltransferase over-expression. This is a causative event in multiple myeloma, underscoring the significance of histone modifications.

[2006-2009] Biosciences, University of Birmingham. Dr Helen Cooper and Professor John Heath.

Src-dependent protein interactions and tyrosine phosphorylation in the FGF pathway were identified using SILAC relative quantification. Electron capture dissociation was applied to the on-line identification and site-localization of protein phosphorylation, using both targeted and proteomic approaches. Site-localization software (SLoMo) was developed to analyse the phosphoproteomic dataset.

PhD

[2002-2005] Phosphoprotein Analysis by Mass Spectrometry

Department of Chemistry, The University of Manchester. Professor Simon Gaskell.

My PhD focussed on novel method development, including phosphopeptide enrichment using SCX-based diagonal chromatography and TiO2. Novel phosphorylation sites were identified from the TGF-β receptor and quantified using iTRAQ.

MSci

[2001-2002] Department of Biochemistry, University of Cambridge. Dr Sarah Lummis.

The ligand specificity of the 5HT3-R was investigated using site-directed mutagenesis, radioligand-binding assays and electrophysiology (patch-clamping).

Role

Principal Investigator in the Genome Damage and Stability Centre.